文献类型：期刊文献

中文题名：多年生黑麦草愈伤组织诱导和植株再生

英文题名：Callus Induction and Plant Regeneration of Perennial Ryegrass

作者：冯霞[1] 孙振元[1] 韩蕾[1] 彭镇华[1]

第一作者：冯霞

机构：[1]中国林业科学研究院生物技术实验室

年份：2004

卷号：21

期号：10

起止页码：23-28

中文期刊名：草业科学

外文期刊名：Pratacultural Science

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：国家转基因植物研究与产业化专项 (J 2 0 0 2 B 0 0 6)

语种：中文

中文关键词：多年生黑麦草;愈伤组织;组织诱导;植株再生

外文关键词：perennial ryegrass; mature seeds; tissue culture; plant regeneration

分类号：S543.6

摘要：对多年生黑麦草Lolium perenne品种“德比”成熟种子的愈伤组织诱导和植株再生进行了探索研究。结果表明 :在附加 10mg/L 2 ,4 D ,5 0 0mg/LCH(水解酪蛋白 ) ,0 5mg/L 6 BA和 0 5mg/LNAA的N6培养基中愈伤诱导率最高 ,达 85 %。分化培养基以附加 1mg/L、6 BA、0 5mg/LKT的N6培养基为最优 ,分化率为 77 73%。在大量元素减半的N6培养基中附加 30g/L蔗糖及 0 2mg/LNAA进行生根培养 ,生根率达 10 0 %。
The objective of this study was to optimize tissue culture responses of turf-type perennial ryegrass (Lolium perenne L.) for genetic transformation, the effects of medium supplements on tissue culture responses were investigated with mature seeds of an elite cultivar, “Derby”, as explant. The best callus induction was obtained in N 6 medium with 10 mg/L 2,4-D, 500 mg/L CH, 0.5 mg/L 6-BA and 0.5 mg/L NAA, of which the induction rate was 85%. The highest differentiation rate, (77.13%), was achieved in the medium contained N 6 medium and 1 mg/L 6-BA, 0.5 mg/L KT. The root inducing medium, containing 0.2 mg/L NAA , 30 g/L Sucrose and N6 medium with half macro, gave 100% root inducing rate.