文献类型：期刊文献

英文题名：Morphological and Comparative Transcriptome Analysis of Three Species of Five-Needle Pines: Insights Into Phenotypic Evolution and Phylogeny

作者：Li, Xiang[1] Cai, Kewei[1] Zhao, Qiushuang[1] Li, Hanxi[1] Wang, Xuelai[1] Tigabu, Mulualem[2] Sederoff, Ronald[3] Ma, Wenjun[4] Zhao, Xiyang[1,5]

第一作者：Li, Xiang

通信作者：Zhao, XY[1];Ma, WJ[2];Zhao, XY[3]|[a0005305bd58d3ae0e53e]赵曦阳;

机构：[1]Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China;[2]Swedish Univ Agr Sci, Fac Forest Sci, Southern Swedish Forest Res Ctr, Lomma, Sweden;[3]North Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC USA;[4]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[5]Jilin Agr Univ, Coll Forestry & Grassland, Changchun, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85125235147);WOS:【SCI-EXPANDED(收录号:WOS:000760785700001)】；

基金：The authors would like to thank the members of the State Key Laboratory of Tree Genetics and Breeding for their assistance during laboratory works and for fruitful discussions.

语种：英文

外文关键词：five-needle pines; Pinus koraiensis; Pinus sibirica; Pinus pumila; transcriptome sequencing; SSRs; Pinus phylogeny

摘要：Pinus koraiensis, Pinus sibirica, and Pinus pumila are the major five-needle pines in northeast China, with substantial economic and ecological values. The phenotypic variation, environmental adaptability and evolutionary relationships of these three five-needle pines remain largely undecided. It is therefore important to study their genetic differentiation and evolutionary history. To obtain more genetic information, the needle transcriptomes of the three five-needle pines were sequenced and assembled. To explore the relationship of sequence information and adaptation to a high mountain environment, data on needle morphological traits [needle length (NL), needle width (NW), needle thickness (NT), and fascicle width (FW)] and 19 climatic variables describing the patterns and intensity of temperature and precipitation at six natural populations were recorded. Geographic coordinates of altitude, latitude, and longitude were also obtained. The needle morphological data was combined with transcriptome information, location, and climate data, for a comparative analysis of the three five-needle pines. We found significant differences for needle traits among the populations of the three five-needle pine species. Transcriptome analysis showed that the phenotypic variation and environmental adaptation of the needles of P. koraiensis, P. sibirica, and P. pumila were related to photosynthesis, respiration, and metabolites. Analysis of orthologs from 11 Pinus species indicated a closer genetic relationship between P. koraiensis and P. sibirica compared to P. pumila. Our study lays a foundation for genetic improvement of these five-needle pines and provides insights into the adaptation and evolution of Pinus species.