文献类型：期刊文献

英文题名：ycf1, the most promising plastid DNA barcode of land plants

作者：Dong, Wenpan[1] Xu, Chao[1] Li, Changhao[1,2] Sun, Jiahui[1,2] Zuo, Yunjuan[1] Shi, Shuo[1] Cheng, Tao[1] Guo, Junjie[3] Zhou, Shiliang[1]

第一作者：Dong, Wenpan

通信作者：Zhou, SL[1]

机构：[1]Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2015

卷号：5

起止页码：8348

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000349245100001)】；

基金：This study was partly supported by grants from the Ministry of Science and Technology of China (2011FY120200, 2012BAC01B05 & 2012AA021602) and National Natural Science Foundation of China (31270239). We are grateful to Hongmei Liu, Cunming Chen, Lei Shi, Jun Wen, Ling Wang, Yi Zhong, Xu Quan, Fangfang Zhao, Ping Long, Mu Liu, and Jianhua Xue for their help in field and laboratory work, and/or provision of plant materials.

语种：英文

摘要：A DNA barcode is a DNA fragment used to identify species. For land plants, DNA fragments of plastid genome could be the primary consideration. Unfortunately, most of the plastid candidate barcodes lack species-level resolution. The identification of DNA barcodes of high resolution at species level is critical to the success of DNA barcoding in plants. We searched the available plastid genomes for the most variable regions and tested the best candidates using both a large number of tree species and seven well-sampled plant groups. Two regions of the plastid gene ycf1, ycf1a and ycf1b, were the most variable loci that were better than existing plastid candidate barcodes and can serve as a barcode of land plants. Primers were designed for the amplification of these regions, and the PCR success of these primers ranged from 82.80% to 98.17%. Of 420 tree species, 357 species could be distinguished using ycf1b, which was slightly better than the combination of matK and rbcL. For the well-sampled representative plant groups, ycf1b generally performed better than any of the matK, rbcL and trnH-psbA. We concluded that ycf1a or ycf1b is the most variable plastid genome region and can serve as a core barcode of land plants.