文献类型：期刊文献

英文题名：Transcriptomic Profiling of Buds Unveils Insights into Floral Initiation in Tea-Oil Tree (Camellia oleifera 'changlin53')

作者：Guo, Hongyan[1] Zhou, Zongshun[1] Zhou, Jian[2] Yan, Chao[1] Zhong, Wenbin[1] Li, Chang[3] Jiang, Ying[1] Yuan, Yaqi[1] Cao, Linqing[1] Pan, Wenting[1] Wang, Jinfeng[1] Wang, Jia[1] He, Tieding[1] Hua, Yikai[1] Liu, Yisi[1] Cao, Lixian[1] Chen, Chuansong[1]

第一作者：郭红艳

通信作者：Chen, CS[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Key Lab Cultivat & Utilizat Oil Camellia Resources, Xinyu 336600, Peoples R China;[2]Xinyu Univ, Xinyu 336600, Peoples R China;[3]Jiangxi Environm Engn Vocat Coll, Ganzhou 341000, Peoples R China

年份：2025

卷号：14

期号：15

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-105013108819);WOS:【SCI-EXPANDED(收录号:WOS:001548721300001)】；

基金：This research study was funded by the Jiangxi Provincial Natural Science Foundation, grant number 20232BAB215050, and the APC was funded by the Jiangxi Provincial Natural Science Foundation.

语种：英文

外文关键词：Camellia oleifera; floral initiation; buds; transcriptome analysis; gibberellins

摘要：Flowering is a key agronomic trait that directly influences the yield of the tea-oil tree (Camellia oleifera). Floral initiation, which precedes flower bud differentiation, represents a critical developmental stage affecting the flowering outcomes. However, the molecular mechanisms underlying floral initiation in C. oleifera remain poorly understood. In this study, buds from five key developmental stages of a 12-year-old C. oleifera cultivar 'changlin53' were collected as experimental samples. Scanning electron microscopy was employed to identify the stage of floral initiation. UPLC-MS/MS was used to analyze endogenous gibberellin (GA) concentrations, while transcriptomic analysis was performed to reveal the underlying transcriptional regulatory network. Six GA types were detected during floral initiation and petal development. GA4 was exclusively detected at the sprouting stage (BII), while GA3 was present in all samples but was significantly lower in BII and the flower bud primordium formation stage (BIII) than in the other samples. A total of 64 differentially expressed genes were concurrently enriched in flower development, reproductive shoot system development, and shoot system development. Weighted gene co-expression network analysis (WGCNA) identified eight specific modules significantly associated with different developmental stages. The magenta module, containing Unigene0084708 (CoFT) and Unigene0037067 (CoLEAFY), emerged as a key regulatory module driving floral initiation. Additionally, GA20OX1 and GA2OX8 were identified as candidate genes involved in GA-mediated regulation of floral initiation. Based on morphological and transcriptomic analyses, we conclude that floral initiation of C. oleifera is a continuous regulatory process governed by multiple genes, with the FT-LFY module playing a central role in the transition from apical meristem to floral meristem.