文献类型：期刊文献

英文题名：A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa

作者：Lin, Chien-Yuan[1,2] Li, Quanzi[3] Tunlaya-Anukit, Sermsawat[4] Shi, Rui[1] Sun, Ying-Hsuan[5] Wang, Jack P.[1,6] Liu, Jie[1] Loziuk, Philip[7] Edmunds, Charles W.[8] Miller, Zachary D.[8] Peszlen, Ilona[8] Muddiman, David C.[7] Sederoff, Ronald R.[1] Chiang, Vincent L.[1,6]

第一作者：Lin, Chien-Yuan

通信作者：Sederoff, RR[1];Chiang, VL[1];Chiang, VL[2]

机构：[1]N Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC 27695 USA;[2]Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 USA;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]SCG Packaging PLC, 19 Moo 19,Saeng Xuto Rd, Ban Pong 70110, Ratchaburi, Thailand;[5]Natl Chung Hsing Univ, Dept Forestry, Taichung 40227, Taiwan;[6]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[7]N Carolina State Univ, Dept Chem, WM Keck FTMS Lab, Raleigh, NC 27695 USA;[8]N Carolina State Univ, Dept Forest Biomat, Raleigh, NC 27695 USA

年份：2016

卷号：12

期号：2

外文期刊名：TREE GENETICS & GENOMES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000372272300008)】；

基金：The National Science Foundation (USA), Plant Genome Research Program (Grant DBI-0922391) to V.L.C., the NC State University Jordan Family Endowment, NIH/NCSU Molecular Biotechnology Training Program (Grant 5T32GM00-8776-08), and the NC State University Forest Biotechnology Industrial Research Consortium supported this work.

语种：英文

外文关键词：Lignin polymerization; Populus trichocarpa; Lignin peroxidase; LC-MS/MS; Lignin systems biology

摘要：Class III peroxidases are members of a large plant-specific sequence-heterogeneous protein family. Several sequence-conserved homologs have been associated with lignin polymerization in Arabidopsis thaliana, Oryza sativa, Nicotiana tabacum, Zinnia elegans, Picea abies, and Pinus sylvestris. In Populus trichocarpa, a model species for studies of wood formation, the peroxidases involved in lignin biosynthesis have not yet been identified. To do this, we retrieved sequences of all PtrPOs from Peroxibase and conducted RNA-seq to identify candidates. Transcripts from 42 PtrPOs were detected in stem differentiating xylem (SDX) and four of them are the most xylem-abundant (PtrPO12, PtrPO21, PtrPO42, and PtrPO64). PtrPO21 shows xylem-specific expression similar to that of genes encoding the monolignol biosynthetic enzymes. Using protein cleavage-isotope dilution mass spectrometry, PtrPO21 is detected only in the cell wall fraction and not in the soluble fraction. Downregulated transgenics of PtrPO21 have a lignin reduction of similar to 20 % with subunit composition (S/G ratio) similar to wild type. The transgenics show a growth reduction and reddish color of stem wood. The modulus of elasticity (MOE) of the stems of the downregulated PtrPO21-line 8 can be reduced to similar to 60 % of wild type. Differentially expressed gene (DEG) analysis of PtrPO21 downregulated transgenics identified a significant overexpression of PtPrx35, suggesting a compensatory effect within the peroxidase family. No significant changes in the expression of the 49 P. trichocarpa laccases (PtrLACs) were observed.