文献类型：期刊文献

英文题名：RAPD and morphological diversity among four populations of the tropical tree species Paramichelia baillonii (Pierre) Hu in China

作者：Li, Fagen[1] Gan, Siming[1] Weng, Qijie[1] Zhao, Xia[1] Huang, Shineng[1] Li, Mei[1] Chen, Shaoyu[2] Wang, Qinghua[2] Shi, Fuqiang[2]

第一作者：李发根

通信作者：Gan, S.

机构：[1] Research Institute of Tropical Forestry, Chinese Academy of Forestry, Longdong, Guangzhou, 510520 Guangdong Province, China; [2] Yunnan Academy of Forestry, Heilongtan, Kunming 650204, Yunnan Province, China

年份：2008

卷号：255

期号：5-6

起止页码：1793-1801

外文期刊名：Forest Ecology and Management

收录：EI(收录号：20081011132705);Scopus(收录号：2-s2.0-39849094890)

语种：英文

外文关键词：Biodiversity - Biomarkers - Conservation - Morphology - Population dynamics - Principal component analysis

摘要：Genetic variation and structure of four natural populations of Paramichelia baillonii (Pierre) Hu (family Magnoliaceae) from China were assessed using random amplified polymorphic DNA (RAPD) markers and morphological characters. For RAPD analysis, 17 primers generated a total of 152 discernible and reproducible bands across the four populations analyzed, out of which 63 (41.4%) were polymorphic. The Shannon's index (Ho) varied with population, ranging from 0.555 to 1.121, with an average value of 0.955. The mean diversity at species level was Hsp = 1.217. Most of the genetic variation was found within population although pair-wise ΦST values and Bartlett's test indicated significant differentiation among populations. The between-population component comprised 21.1% and 15.7% of the total genetic variation, respectively, as assessed by the Shannon's index and AMOVA analysis. The gene flow (Nm) ranged from a moderate value of 0.464 (between populations YL and YE) to a high value of 4.128 (between populations YP and YE), with an average value of 1.570. The results for P. baillonii are generally in accordance with those already recorded for other outcrossing tropical tree species. For morphological characters, principal component analysis showed that the first two components explained a total of 77.6% of the whole phenotypic variability, but a dispersion plot of all the families defined by the first two principal components did not show any significant grouping. The overall population differentiation index in quantitative traits (QST) is 0.07 and less than that at RAPD markers (ΦST, 0.157). The pair-wise QST estimates are significantly correlated with pair-wise ΦST (r = 0.8643, P = 0.0264) while the correlation between the Euclidean distance matrices based on morphological and RAPD data was low and non-significant (r = -0.0189, P = 0.3103). The information obtained here could be valuable for devising strategies for conservation and domestication of P. baillonii. ? 2007 Elsevier B.V. All rights reserved.