文献类型：期刊文献

中文题名：The phased chromosome-scale genome of yellowhorn sheds light on the mechanism of petal color change

作者：Quanxin Bi[1] Mengke Wang[1] Jiang Li[2] Jinmin Lian[2] Yongchao Niu[2] Yifan Cui[1] Xiaojuan Liu[1] Haiyan Yu[1] Libing Wang[1]

第一作者：毕泉鑫

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;[2]Biozeron Shenzhen,Inc.,Shenzhen,Guangzhou,Guangdong 518000,China

年份：2023

卷号：9

期号：6

起止页码：1193-1206

中文期刊名：Horticultural Plant Journal

外文期刊名：园艺学报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2023_2024】；

基金：supported by the Central PublicInterest Scientific Institution Basal Research Fund (Grant No. CAFYBB2020QB001);the National Natural Science Foundation of China (Grant No. 31800571)。

语种：英文

中文关键词：Xanthoceras sorbifolium var.purpurea;Phased genome;Metabolomics;Transcriptomics;Petal color

分类号：R73

摘要：Yellowhorn(Xanthoceras sorbifolium), especially its varieties, is the red petal yellowhorn(X. sorbifolium var. purpurea), an important tree species with great ornamental value, and its flower petals change color throughout the flowering period. In this study, we mainly focused on the mechanism of the petal color change with transcriptomics and metabolomics data. A phased chromosome-scale assembly of the red petal yellowhorn genome was generated using the PacBio high-fidelity reads, Illumina short reads, and Phase genomics Proximo Hi-C data. The final de novo assembly yielded 533.67 Mb with a contig N50 of 5.42 Mb, and 27 501 protein-coding genes were predicted. Notably, an alternate haplotig assembly was also obtained. Furthermore, a variation database for the alleles within the genome was constructed. Subsequently, the expression pattern of flower pigmentation-related genes and allelic expression imbalance events were investigated. Apart from 6 genes involved in the anthocyanin biosynthesis pathway regulated by the activation of 15 MYB-bHLH-WD40s, the increased expression of senescencerelated gene 1(SRG1) and 2-oxoglutarate-dependent dioxygenase(DIOX5) might also result in decreasing content of lutein and increasing abundance of(E/Z)-phytoene, cyanidin-3-O-rutinoside, and cyanidin-3-O-sambubioside. These changes in genes and metabolites were most likely related to the petal color change in red petal yellowhorn. This phased chromosome-scale genome assembly provides more accurate genomic information for future molecular breeding and facilitates allele function studies of the red petal yellowhorn.