文献类型：期刊文献

英文题名：Overexpression of PagLAR3 in Populus alba x P. glandulosa Promotes Resistance to Hyphantria cunea

作者：Fan, Zhibin[1] Hou, Luxuan[1] Wang, Zheshu[1] Wang, Lijuan[1,2]

第一作者：Fan, Zhibin

通信作者：Wang, LJ[1];Wang, LJ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat Natl Forestry, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China

年份：2025

卷号：15

期号：6

外文期刊名：AGRONOMY-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001515159500001)】；

基金：This work was supported by Biological Breeding-National Science and Technology Major Project (2022ZD04015).

语种：英文

外文关键词：Populus alba x P. glandulosa; PagLAR3; resistance; Hyphantria cunea

摘要：Poplar is a vital ecological and economic tree species. In recent years, poplar plantations in China have been increasingly threatened by the fall webworm (Hyphantria cunea). Developing resistant varieties through genetic engineering is an environmentally friendly and cost-effective approach to controlling this pest. Although some exogenous toxic genes have been used in insect-resistant poplar breeding, endogenous defense genes remain scarce. This study focused on tannins, key defensive metabolites in poplar, and explored the role of PagLAR3, a gene encoding a crucial enzyme in condensed tannin biosynthesis, in poplar's defense against the fall webworm. The findings revealed that overexpression of PagLAR3 significantly increased levels of catechin, gallocatechin, procyanidin B3, and procyanidin C2 in poplar leaves. Feeding assays with fall webworm larvae demonstrated that, compared with an 84 K (P. alba x P. glandulosa) control, transgenic lines overexpressing PagLAR3 significantly reduced larval and pupal weight, prolonged larval duration, and caused a decrease in adult emergence. Development retardation caused by overexpression of PagLAR3 in fall webworm is expected to effectively control the pest population, thereby mitigating damage to poplar trees. PagLAR3 represents a potential target for enhancing poplar resistance to the fall webworm.