文献类型：期刊文献

英文题名：Role of cin-miR2118 in drought stress responses in Caragana intermedia and Tobacco

作者：Wu, Bi-fei[1] Li, Wan-feng[2] Xu, Hai-yan[2] Qi, Li-wang[2] Han, Su-ying[1]

第一作者：Wu, Bi-fei

通信作者：Han, SY[1]

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2015

卷号：574

期号：1

起止页码：34-40

外文期刊名：GENE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000364246900005)】；

基金：This work was supported by the National High Technology Research and Development Program of China (2011AA100203) and the National Natural Science Foundation of China (30830086).

语种：英文

外文关键词：Cin-miR2118; TIR-NBS-LRR; Caragana intermedia; Drought tolerance

摘要：The miR2118 is highly conserved in leguminous plants. Its function is to regulate the expression of genes encoding the TIR-NBS-LRR resistance protein. In this study, cin-miR2118 from Caragana intermedia was functionally characterized, especially with regard to its role in drought stress resistance. Two target genes of cin-miR2118 were predicted and cloned, the occurrence of miR2118 target sequence in both genes indicated that they might be targets of cin-miR2118. We investigated the expression patterns of cin-miR2118 and its target genes in C intermedia stems and found diverse changes in expression in response to drought stress. CiDR1 was negatively correlated with corresponding miR2118 expression while CiDR2 was positively correlated with cin-miR2118. For further study, induced tolerance was observed in the transgenic Tobacco with overexpression cin-miR2118 upon 140-min water deficiency. And the expression level of cin-miR2118 was dramatically increased under drought stress. These results reveal that cin-miR2118 exert positive effects on drought stress tolerance. In addition, our study unexpectedly found that overexpression of cin-miR2118 in Tobacco can cause phenotype changes, which suggested that cin-miR2118 may have a novel function as a growth regulator in Tobacco. (C) 2015 Elsevier B.V. All rights reserved.