文献类型：期刊文献

英文题名：Efficient production of isoquercitin, icariin and icariside II by a novel thermostable α-l-rhamnosidase PodoRha from Paenibacillus odorifer with high α-1, 6-/α-1, 2- glycoside specificity

作者：Xie, Jingcong[1,2] Zhao, Jian[1,2] Zhang, Ning[1,2] Xu, Hao[1,2] Yang, Jing[1,2] Ye, Jun[1,2] Jiang, Jianchun[1,2]

第一作者：Xie, Jingcong

机构：[1] Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, National Engineering Lab. for Biomass Chemical Utilization, Key and Open Lab of Forest Chemical Engineering, SFA, Key Lab of Biomass Energy Sources and Materials, Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grass and Administration, Nanjing, 210042, China; [2] Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China

年份：2022

卷号：158

外文期刊名：Enzyme and Microbial Technology

收录：EI(收录号：20221812067889);Scopus(收录号：2-s2.0-85129271143)

基金：This work was financially supported by Fundamental Research funds of CAF ( CAFYBB2018GD002-04 ), National Natural Science Foundation of China (No. 32101477 ), Jiangsu Key Laboratory of Biomass Energy and Material (No. JSBEM-S-201802 ), and National Natural Science Foundation of China (No. 31770636 ).

语种：英文

外文关键词：Alcohols - Biochemistry - Cloning - Escherichia coli - Productivity - Stability - Sugars

摘要：Minor flavonoids, such as isoquercitin, icariin and icariside II, have desired bioactivity and bioavailability. To enhance the production efficiency on converting rutin and epimedin C into minor flavonoids, a novel GH family 78 α-L-rhamnosidase PodoRha from the Paenibacillus odorifer was cloned and heterologous over-expressed in E. coli BL21(DE3). PodoRha exhibited a high catalytic ability in cleaving outer α-1, 2-rhamnopyranosyl moieties of epimedin C and α-1, 6- rhamnopyranosyl of rutin. It displayed the highest activity at 45 °C and pH 6.5, and has great thermostability at 40 °C. Under the optimal transformation condition (40 °C, pH 6.5, 1 h incubation), 10 g/L of Rutin was 99.86% transformed into 6.95 g/L of isoquercitin by PodoRha with a crude productivity of 6.95 g·L-1·h-1; 1.964 g/L icariin was detected after the epimedin C of the total Epimedium flavonoids (10 g/L) were 99.98% converted by PodoRha. Moreover, by using a two-stage conversion system with a crude icariside II productivity of 2.081 g·L-1·h-1, 5 g/L epimedin C was 99.85% converted into 3.122 g/L of icariside II with 1 h incubation under pH 6.5 at 40 °C after addition of 5.5 U/mL PodoRha; then with 30 min incubation under pH 6.5 at 90 °C after addition of 0.8 U/mL IagBgl1. ? 2022 Elsevier Inc.