文献类型：期刊文献

英文题名：Characteristics of Community Structure and Functional Group of Fungi in Leaf, Stem, Root and Rhizosphere Soil of Ligustrum lucidum

作者：Bin, Li[1] Hongxiang, Shi[2] Lanlan, Liu[2] Pu, Yang[1] Xin, Zhang[1] Hang, Chen[1] Ying, Feng[1] Xiaoming, Chen[1]

机构：[1] Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming, 650224, China; [2] College of Biodiversity Conservation, Southwest Forestry University, Kunming, 650224, China

年份：2022

卷号：58

期号：5

起止页码：102-112

外文期刊名：Linye Kexue/Scientia Silvae Sinicae

收录：EI(收录号：20223912794720)

语种：英文

外文关键词：Forestry - RNA - Soils

摘要：Objective This study aimed to explore the species composition, diversity and functional group characteristics of fungal community in three vegetative organs and rhizosphere soil of Ligustrum lucidum, so as to lay a foundation for the privet - fungi interaction study and the development and utilization of the privet endophytic and rhizosphere fungal resources. This research could provide a reference for the study of trees microbiome. Method The leaf, stem, root and rhizosphere soil of L. lucidum were selected as research objects, and the ITS1 region of fungal rRNA gene was sequenced by Illumina MiSeq instrument. Based on the sequencing result, the diversity, species composition, functional group characteristics and their difference in community structure of fungal community in three vegetative organs and rhizosphere soil were analyzed subsequently. Result The species richness of fungal community of different samples was in the order of RS>SE>RE>LE, and the community diversity was RS>LE>SE>RE. There were 31 OTUs in the four samples, accounting for 2. 08% of the total OTUs. Approximately 39. 42%, 35. 26% and 76. 94% of OTUs in leaves, stems and roots were also found in rhizosphere soil, respectively. The β diversity analysis showed that the fungal community structure of leaf was relatively similar to that of stem, but was different from that of root and rhizosphere soil. At the phylum level, the dominant taxon of fungi in the four compartments were Ascomycota and Basidiomycota. At the genus level, the dominant fungi of endophytic fungi community in leaves and stems were Cladosporium and Vishniacozyma while the dominant fungi in the root were Rhexodenticula and Pochonia. In addition, the dominant fungi of rhizosphere soil were Psilocybe and Saitozyma. The common fungi in leaves, stems, roots and rhizosphere soil mainly were Cladosporium, Vishniacozyma, Saitozyma, Rachicladosporium, Symmetrospora, Alternaria and Penicillium. The statistical result of fungi functional group classification showed that the differentiation characteristics of samples could be divided into aboveground and underground parts with the fungi in leaves and stems were mainly of compound trophic (pathotrophy-saprotrophy-symbiotrophy, pathotrophy-saprotrophy), which accounted for 76. 97% and 91. 68%, respectively, while those were mainly of single trophic fungi (saprotrophy, symbiotrophy) in root and rhizosphere soil, accounting for 60. 05% and 67. 51%, respectively. ConclusionsThe diversity of fungal community in rhizosphere soil is significantly higher than that in leaves, stems and roots of L. lucidum. The fungal community structure and functional group characteristic of leaves are relatively similar to that of stems, but significantly different from those of roots and rhizosphere soil. The fungi functional group in leaves and stems is mostly pathotrophy - saprotrophy - symbiotrophy, while that in root and rhizosphere soil is mostly saprotrophy. ? 2022 Chinese Society of Forestry. All rights reserved.