文献类型：期刊文献

英文题名：An Empirical Assessment of Transgene Flow from a Bt Transgenic Poplar Plantation

作者：Hu, Jianjun[1,2] Zhang, Jin[1,2] Chen, Xingling[3] Lv, Jinhui[1] Jia, Huixia[1,2] Zhao, Shutang[1,2] Lu, Mengzhu[1,2]

第一作者：胡建军;Hu, Jianjun

通信作者：Lu, MZ[1];Lu, MZ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing, Jiangsu, Peoples R China;[3]Changchun Acad Forestry, Changchun, Peoples R China

年份：2017

卷号：12

期号：1

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000391972600076)】；

基金：This work was supported by the National High-Tech R&D Program of China (2013AA102702 and 2011AA10020101). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank Dr. Andrew Groover (UC-Davis, US Forest Service) and Prof. Xiao-Ru Wang (Umea University) for critical comments on the manuscript and Dr. Chun-Ling Zhang for the contribution of Bt seeds detection. This work was supported by the National High-Tech R&D Program of China (2013AA102702) and (2011AA10020101).

语种：英文

摘要：To assess the possible impact of transgenic poplar plantations on the ecosystem, we analyzed the frequency and distance of gene flow from a mature male transgenic Populus nigra plantation carrying the Bacillus thuringiensis toxin gene (Bt poplar) and the survival of Bt poplar seeds. The resultant Bt poplar seeds occurred at a frequency of similar to 0.15% at 0 m to similar to 0.02% at 500 m from the Bt poplar plantation. The germination of Bt poplar seeds diminished within three weeks in the field (germination rate from 68% to 0%) compared to 48% after three weeks of storage at 4 degrees C. The survival rate of seedlings in the field was 0% without any treatment but increased to 1.7% under the addition of four treatments (cleaning and trimming, watering, weeding, and covering with plastic film to maintain moisture) after being seeded in the field for eight weeks. The results of this study indicate that gene flow originating from the Bt poplar plantation occurred at an extremely low level through pollen or seeds under natural conditions. This study provides first-hand field data on the extent of transgene flow in poplar plantations and offers guidance for the risk assessment of transgenic poplar plantations.