文献类型：期刊文献

英文题名：The role of senescence-associated gene101 (PagSAG101a) in the regulation of secondary xylem formation in poplar

作者：He, Hui[1] Song, Xue-Qin[1] Jiang, Cheng[2] Liu, Ying-Li[1] Wang, Dian[3] Wen, Shuang-Shuang[1] Chai, Guo-Hua[4] Zhao, Shu-Tang[1,5] Lu, Meng-Zhu[1,2,5]

第一作者：He, Hui

通信作者：Zhao, ST[1];Lu, MZ[1];Lu, MZ[2];Zhao, ST[3];Lu, MZ[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Coll Forestry & Biotechnol, Hangzhou 311300, Peoples R China;[3]Qingdao Agr Univ, Coll Agron, Qingdao 266109, Peoples R China;[4]Qingdao Agr Univ, Coll Resources & Environm, Qingdao 266109, Peoples R China;[5]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China

年份：2022

卷号：64

期号：1

起止页码：73-86

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000745086400008)】；

基金：This work was supported by the Fundamental Research Funds of CAF (CAFYBB2017ZY001) and the Ten-thousand Talents Program of China to Meng-Zhu

语种：英文

外文关键词：cambium; PagSAG101a; Populus; secondary cell wall; xylem formation

摘要：Wood is produced by the accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated PagSAG101a, the homolog of Arabidopsis thaliana SAG101, from a hybrid poplar (Populus alba x Populus glandulosa) clone 84K and investigated its role in secondary xylem development. PagSAG101a was expressed predominantly in lignified stems and localized in the nucleus. Compared with non-transgenic 84K plants, transgenic plants overexpressing PagSAG101a displayed increased plant height, internode number, stem diameter, xylem width, and secondary cell wall thickness, while opposite phenotypes were observed for PagSAG101a knock-out plants. Transcriptome analyses revealed that differentially expressed genes were enriched for those controlling cambium cell division activity and subsequent secondary cell wall deposition during xylem formation. In addition, the tandem CCCH zinc finger protein PagC3H17, which positively regulates secondary xylem width and secondary wall thickening in poplar, could bind to the promoter of PagSAG101a and mediate the regulation of xylem differentiation. Our results support that PagSAG101a, downstream of PagC3H17, functions in wood development.