文献类型：期刊文献

英文题名：Screening and a Comprehensive Evaluation of Pinus elliottii with a High Efficiency of Phosphorus Utilization

作者：Liu, Huan[1,2] He, Zhengquan[1] Yang, Yuying[2] Zhao, Yazhi[1,2] Chen, Huiling[1,2] Chen, Shuxin[2] Wu, Shaoze[2] Luan, Qifu[2] Zhuo, Renying[2] Han, Xiaojiao[2]

第一作者：Liu, Huan

通信作者：Han, XJ[1]

机构：[1]China Three Gorges Univ, Biotechnol Res Ctr, Key Lab Three Gorges Reg Plant Genet & Germplasm E, Yichang 443002, Peoples R China;[2]Chinese Acad Forestry, Zhejiang Key Lab Forest Genet & Breeding, State Key Lab Tree Genet & Breeding, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2025

卷号：16

期号：8

外文期刊名：FORESTS

收录：;EI(收录号：20253519080852);Scopus(收录号：2-s2.0-105014400951);WOS:【SCI-EXPANDED(收录号:WOS:001560162200001)】；

基金：Supported by the major projects in scientific innovation, Ministry of Science and Technology, the People's Republic of China (2023ZD0405805) to X.H.

语种：英文

外文关键词：Pinus elliottii; line selection; low-phosphorus tolerance; phosphorus utilization efficiency

摘要：To investigate the responses and mechanisms of slash pine under low orthophosphate (Pi) stress and to identify Pi-efficient lines, we analyzed 12 indices related to biomass, root traits, and tissue Pi concentration across 13 slash pine lines subjected to varying Pi treatments. The composite assessment value of low-phosphorus tolerance (D) was calculated by evaluating these 12 response indicators through principal component analysis, in conjunction with the fuzzy membership function method. Nine low-phosphorus tolerance factors (LPTFs)-including above-ground fresh weight (0.69), below-ground fresh weight (0.52), total root length (0.56), root surface area (0.63), root volume (0.67), above-ground Pi concentration (0.78), below-ground Pi concentration (0.52), bioconcentration factor (0.77), and P utilization efficiency (-0.76)-showed significant correlations with D (p < 0.05). Utilizing these nine LPTFs, cluster analysis classified the 13 lines into the following three groups according to their low-phosphorus (P) tolerance: high-P-efficient, medium-P-efficient, and low-P-efficient lines. Under low Pi and Pi-deficiency treatments, line 27 was identified as a high-P-efficient line, while lines 1, 6, and 9 were classified as low-P-efficient lines. Notably, eight genes (SPX1, SPX3, SPX4, PHT1;1, PAP23, SQD1, SQD2, NPC4) and five genes (SPX1, SPX3, SPX4, PAP23, SQD1) were significantly up-regulated in the roots and leaves of both line 27 and line 9 under low-phosphorus stress, respectively. However, the high-P-efficient line 27 exhibited a stronger regulatory capacity with a higher expression of two genes (SPX4, SQD2) in the roots and nine genes (SPX1, SPX3, SPX4, PHT1;1, PAP10, PAP23, SQD1, SQD2, NPC4) in the leaves under low Pi stress. These findings reveal differential responses to low Pi stress among slash pine lines, with line 27 displaying superior low-P tolerance, enabling better adaptation to low Pi environments and the maintenance of normal growth, development, and physiological activities.