文献类型：期刊文献

英文题名：Identification and analysis of slow anion channel proteins (SLACs) gene families involved in drought stress in Orchidaceae

作者：Peng, Fu-Cheng[1] Yuan, Meng[1] Hou, Tian-Ze[1] Zhou, Lin[1] Zheng, Bao-Qiang[1] Wang, Yan[1]

第一作者：Peng, Fu-Cheng

机构：[1] Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China

年份：2025

卷号：231

外文期刊名：Industrial Crops and Products

收录：EI(收录号：20251918390397);Scopus(收录号：2-s2.0-105004552259)

语种：英文

外文关键词：Algae - Fungi - Plant extracts - Plant shutdowns - Transcription - Vegetation

摘要：Slow anion channel proteins (SLACs) play a crucial role in stomatal regulation in response to drought stress in plants. Orchids, especially medicinal orchids, have medical and health benefits, and their secondary components (e.g., polysaccharides, alkaloids, etc.) can be used in the production of various industrial products. Drought stress is a key factor limiting the growth of orchid plants, and there is a lack of research on drought stress-related genes in orchid plants. In this study, we identified 40 genes coding for SLAC proteins from the genomes of seven orchid species and analyzed them for basic protein information, structural features, chromosomal localization, promoter elements, collinear analysis, and phylogenetic relationships. We analyzed the expression pattern of the Dendrobium catenatum SLAC gene family. We found that DcaSLAC1 is mainly expressed in leaves and is induced by abscisic acid (ABA), and its encoded protein is localized to the plasma membrane. Functional complementation experiments with the Arabidopsis atslac1 mutant showed that DcaSLAC1 could backfill the stomatal closure behavior of atslac1 that could not be induced by darkness, Ca2 +, and ABA. Heterologous overexpression of DcaSLAC1 in Arabidopsis (Columbia background) enhanced the drought tolerance of the plants. In addition, virus-induced gene silencing (VIGS) of the DcaSLAC1 gene increased stomatal opening rate and stomatal conductance. This study provides important information for further resolving the functions of orchid SLACs and helps to accumulate valuable genetic resources for breeding of D. catenatum for stress tolerance. ? 2025 The Authors