文献类型：期刊文献

英文题名：Overexpression of cysteine protease gene from Salix matsudana enhances salt tolerance in transgenic Arabidopsis

作者：Zheng, Liu[1,2,3] Chen, Shuangshuang[2,3] Xie, Lihua[2,3] Lu, Zhuchou[1] Liu, Mingying[2,3] Han, Xiaojiao[2,3] Qiao, Guirong[2,3] Jiang, Jing[2,3] Zhuo, Renying[2,3] Qiu, Wenmin[2,3] He, Zhengquan[1]

第一作者：Zheng, Liu

通信作者：He, ZQ[1];Qiu, WM[2]

机构：[1]China Three Gorges Univ, Key Lab Three Gorges Reg Plant Genet & Germplasm, Biotechnol Res Ctr, Yichang 443002, Hubei, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2018

卷号：147

起止页码：53-62

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000424181300006)】；

基金：This work was supported by the Biotechnology Research Center, China Three Gorges University, Yichang, China (No. 2016KBC05), the National Natural Science Foundation of China (No. 31370600 and 31300508), the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (No. 2016C02056-1), the National Nonprofit Institute Research Grant of CAF (No. RISF2014010 and CAFYBB2014QB014).

语种：英文

外文关键词：Cysteine protease; Salix matsudana; Salt stress; Antioxidant

摘要：Salix matsudana is a salt-tolerant arbor tree species. We are interested in understanding its mechanisms of salt-tolerance. Here, we isolated a full-length 1080-bp cDNA for a salt stress-responsive cysteine protease gene from S. matsudana, designated SmCP. The deduced 359-amino-acid SmCP protein was essentially identical to cysteine proteases of other plant species and was predicted to contain inhibitor-I29 and peptidase-C1A domains with three active catalytic sites found in eukaryotic cysteine proteases. Expression profile analysis revealed that SmCP expression is constitutive and overexpression in roots takes place under salt stress. Expression of SmCP in Escherichia coli cells led to enhanced salinity tolerance. In addition, transgenic Arabidopsis thaliana lines over expressing SmCP displayed enhanced tolerance to salt stress, with increased germination rates, antioxidant enzyme (superoxide dismutase) activity, chlorophyll content and ion flux in the root, and reduced malondialdehyde (MDA) levels and electric conductivity compared with control plants. These results indicate that SmCP likely plays an important role in salt tolerance in S. matsudana.