文献类型：期刊文献

英文题名：Tamarix hispida NAC Transcription Factor ThNAC4 Confers Salt and Drought Stress Tolerance to Transgenic Tamarix and Arabidopsis

作者：Mijiti, Meiheriguli[1] Wang, Yucheng[2] Wang, Liuqiang[3] Habuding, Xugela[1]

第一作者：Mijiti, Meiheriguli

通信作者：Habuding, X[1]

机构：[1]Xinjiang Normal Univ, Xinjiang Key Lab Special Species Conservat & Regu, Key Lab Plant Stress Biol Arid Land, Coll Life Sci,Key Lab Special Environm Biodivers, Urumqi 830054, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2022

卷号：11

期号：19

外文期刊名：PLANTS-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000866944800001)】；

基金：The research was supported by grants from the National Natural Science Foundation of China (No.31270703 and 31600539), Key Laboratory Foundation of Xinjiang Normal University (XJNUSYS112018B05), and the "13th Five-Year" Plan for Key Discipline Biology of Xinjiang Normal University.

语种：英文

外文关键词：abiotic stress; Tamarix hispida; ROS scavenging; NAC transcription factor

摘要：Salt and drought are considered two major abiotic stresses that have a significant impact on plants. Plant NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) have been shown to play vital roles in plant development and responses to various abiotic stresses. ThNAC4, a NAC gene from Tamarix hispida involved in salt and osmotic stress tolerance, was identified and characterized in this study. According to a phylogenetic study, ThNAC4 is a member of NAC subfamily II. Subcellular localization analysis showed that ThNAC4 is located in the nucleus, and transcriptional activation experiments demonstrated that ThNAC4 is a transcriptional activator. Transgenic Arabidopsis plants overexpressing ThNAC4 exhibited improved salt and osmotic tolerance, as demonstrated by improved physiological traits. ThNAC4-overexpressing and ThNAC4-silenced T. hispida plants were generated using the transient transformation method and selected for gain- and loss-of-function analysis. The results showed that overexpression of ThNAC4 in transgenic Tamarix and Arabidopsis plants increased the activities of antioxidant enzymes (SOD, POD, and GST) and osmoprotectant (proline and trehalose) contents under stress conditions. These findings suggest that ThNAC4 plays an important physiological role in plant abiotic stress tolerance by increasing ROS scavenging ability and improving osmotic potential.