文献类型：期刊文献

英文题名：Global Lysine Acetylome Analysis of Desiccated Somatic Embryos of Picea asperata

作者：Xia, Yan[1] Jing, Danlong[1] Kong, Lisheng[2] Zhang, Jianwei[1] OuYang, Fangqun[1] Zhang, Hanguo[3] Wang, Junhui[1] Zhang, Shougong[1]

第一作者：Xia, Yan

通信作者：Wang, JH[1];Zhang, SG[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat,State Key Lab Tr, Beijing, Peoples R China;[2]Univ Victoria, Dept Biol, Ctr Forest Biol, Victoria, BC, Canada;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China

年份：2016

卷号：7

期号：DECEMBER2016

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390383500001)】；

基金：This study was supported by a grant from the China Twelfth Five Year Plan for Science & Technology Support (2012BAD01B01).

语种：英文

外文关键词：lysine acetylome; conifer; somatic embryo; partial desiccation treatment; response to stress

摘要：Partial desiccation treatment (PDT) promotes the germination capacity of conifer somatic embryos. Lysine acetylation (LysAc) is a dynamic and reversible post-translational modification that plays a key role in many biological processes including metabolic pathways and stress response. To investigate the functional impact of LysAc in the response of Picea asperata somatic embryos to PDT, we performed a global lysine acetylome analysis. Here, combining antibody-based affinity enrichment and high-resolution mass spectrometry, we identified and validated 1079 acetylation sites in 556 acetylated proteins from P. asperata somatic embryos during PDT. These data represent a novel large-scale dataset of lysine-acetylated proteins from the conifer family. Intensive bioinformatics analysis of the Gene Ontology of molecular functions demonstrated that lysine-acetylated proteins were mainly associated with binding, catalytic activities, and structural molecular activities. Functional characterization of the acetylated proteins revealed that in the desiccated somatic embryos, LysAc is mainly involved in the response to stress and central metabolism. Accordingly, the majority of these interacting proteins were also highly enriched in ribosome, proteasome, spliceosome, and carbon metabolism clusters. This work provides the most comprehensive profile of LysAc for a coniferous species obtained to date and facilitates the systematic study of the physiological role of LysAc in desiccated somatic embryos of P. asperata.