文献类型：期刊文献

英文题名：Genome-Wide Characterization of the BTB Gene Family in Poplar and Expression Analysis in Response to Hormones and Biotic/Abiotic Stresses

作者：Yue, Jing[1] Dai, Xinren[1] Li, Quanzi[1,2] Wei, Mingke[2]

第一作者：Yue, Jing

通信作者：Wei, MK[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100000, Peoples R China;[2]Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China

年份：2024

卷号：25

期号：16

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85202694615);WOS:【SCI-EXPANDED(收录号:WOS:001307002100001)】；

基金：This work was supported by the Fundamental Research Funds of the Chinese Academy of Forestry (CAFYBB2022QC001-2).

语种：英文

外文关键词：Populus trichocarpa; BTB gene family; expression patterns; hormones treatment; biotic/abiotic stresses

摘要：The BTB (Broad-complex, tramtrack, and bric-a-brac) gene family, characterized by a highly conserved BTB domain, is implicated in a spectrum of biological processes, encompassing growth and development, as well as stress responses. Characterization and functional studies of BTB genes in poplar are still limited, especially regarding their response to hormones and biotic/abiotic stresses. In this study, we conducted an HMMER search in conjunction with BLASTp and identified 95 BTB gene models in Populus trichocarpa. Through domain motif and phylogenetic relationship analyses, these proteins were classified into eight families, NPH3, TAZ, Ankyrin, only BTB, BACK, Armadillo, TPR, and MATH. Collinearity analysis of poplar BTB genes with homologs in six other species elucidated evolutionary relationships and functional conservations. RNA-seq analysis of five tissues of poplar identified BTB genes as playing a pivotal role during developmental processes. Comprehensive RT-qPCR analysis of 11 BTB genes across leaves, roots, and xylem tissues revealed their responsive expression patterns under diverse hormonal and biotic/abiotic stress conditions, with varying degrees of regulation observed in the results. This study marks the first in-depth exploration of the BTB gene family in poplar, providing insights into the potential roles of BTB genes in hormonal regulation and response to stress.