文献类型：期刊文献

英文题名：SEMI-ROLLED LEAF 10 stabilizes catalase isozyme B to regulate leaf morphology and thermotolerance in rice (Oryza sativa L.)

作者：Wang, Jiajia[1,2,3] Xu, Jing[1,4] Wang, Li[1] Zhou, Mengyu[1] Nian, Jinqiang[1] Chen, Minmin[1] Lu, Xueli[1] Liu, Xiong[1] Wang, Zian[1] Cen, Jiangsu[1] Liu, Yiting[1] Zhang, Zhihai[1] Zeng, Dali[1] Hu, Jiang[1] Zhu, Li[1] Dong, Guojun[1] Ren, Deyong[1] Gao, Zhenyu[1] Shen, Lan[1] Zhang, Qiang[1] Li, Qing[1] Guo, Longbiao[1] Yu, Sibin[2,3] Qian, Qian[1,5,6] Zhang, Guangheng[1,5,6]

第一作者：Wang, Jiajia

通信作者：Qian, Q[1];Zhang, GH[1];Qian, Q[2];Zhang, GH[2];Qian, Q[3];Zhang, GH[3]

机构：[1]China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou, Peoples R China;[2]Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China;[3]Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Ctr Plant Gene Res, Wuhan, Peoples R China;[4]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Peoples R China;[5]Hainan Yazhou Bay Seed Lab, Sanya, Peoples R China;[6]Chinese Acad Agr Sci, Natl Nanfan Res Inst Sanya, Sanya, Peoples R China

年份：0

外文期刊名：PLANT BIOTECHNOLOGY JOURNAL

收录：;Scopus(收录号：2-s2.0-85146800571);WOS:【SCI-EXPANDED(收录号:WOS:000921428000001)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (32188102, 31861143006 and 31901483), Hainan Provincial key scientific research project, STP program from Hainan Yazhouwan Seed Laboratory (B21HJ0220-02) and Special Support Program(NKYCLJ-C-2021-015) and Nanfan special project (ZDXM06) of CAAS. We thank Prof. Jian Zhang and Dr. Yifeng Wang (CNRRI) for their help in the experiment of protein.

语种：英文

外文关键词：rice (Oryza sativaL.); microRNA; leaf morphology; catalase isozyme B (CATB); thermotolerance

摘要：Plant architecture and stress tolerance play important roles in rice breeding. Specific leaf morphologies and ideal plant architecture can effectively improve both abiotic stress resistance and rice grain yield. However, the mechanism by which plants simultaneously regulate leaf morphogenesis and stress resistance remains elusive. Here, we report that SRL10, which encodes a double-stranded RNA-binding protein, regulates leaf morphology and thermotolerance in rice through alteration of microRNA biogenesis. The srl10 mutant had a semi-rolled leaf phenotype and elevated sensitivity to high temperature. SRL10 directly interacted with catalase isozyme B (CATB), and the two proteins mutually increased one other's stability to enhance hydrogen peroxide (H2O2) scavenging, thereby contributing to thermotolerance. The natural Hap3 (AGC) type of SRL10 allele was found to be present in the majority of aus rice accessions, and was identified as a thermotolerant allele under high temperature stress in both the field and the growth chamber. Moreover, the seed-setting rate was 3.19 times higher and grain yield per plant was 1.68 times higher in near-isogenic line (NIL) carrying Hap3 allele compared to plants carrying Hap1 allele under heat stress. Collectively, these results reveal a new locus of interest and define a novel SRL10-CATB based regulatory mechanism for developing cultivars with high temperature tolerance and stable yield. Furthermore, our findings provide a theoretical basis for simultaneous breeding for plant architecture and stress resistance.