文献类型：期刊文献

英文题名：Overexpression of three orthologous glutathione S-transferases from Populus increased salt and drought resistance in Arabidopsis

作者：Yang, Qi[1,2] Liu, Yan-Jing[1] Zeng, Qing-Yin[1]

第一作者：Yang, Qi

通信作者：Zeng, QY[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, SE-90187 Umea, Sweden

年份：2019

卷号：83

起止页码：57-61

外文期刊名：BIOCHEMICAL SYSTEMATICS AND ECOLOGY

收录：;Scopus(收录号：2-s2.0-85059851447);WOS:【SCI-EXPANDED(收录号:WOS:000461002200014)】；

基金：This work was supported by the National Science Fund for Distinguished Young Scholars (31425006).

语种：英文

外文关键词：Glutathione S-Transferase; Populus; Abiotic stress; Orthologous gene; Arabidopsis

摘要：Glutathione S-transferases (GSTs) are multifunctional proteins and play a role in detoxification of xenobiotics as well as prevention of oxidative damage. This study exogenously overexpressed PtGSTF4 from Populus trichocarpa and its two orthologs from Populus yatungensis and Populus euphratica in Arabidopsis thaliana, respectively. To elucidate the function of three GSTF4 proteins in stress response, we compared germination and seedling growth in transgenic Arabidopsis with salt and drought treatments. All three Populus GSTF4 genes overexpressed Arabidopsis showed enhanced resistance to salt stress and drought. GSTF4 transgenic plants accumulated less hydrogen peroxide and more chlorophylls and decreased levels of lipid peroxidation under salt stress and drought comparing to the mock control plants. The difference observed by GSH and GSSG measurements indicated GSTF4 proteins may involve in glutathione-dependent peroxide scavenging which lead to reduced oxidative damage. The Arabidopsis transformed with the GSTF4 gene form P. euphratica showed higher germination rate and different performance of affecting GSSG contents comparing with the other two orthologous GST genes under NaCl treatment. These results suggested three Populus GSTF4 orthologs may have functional divergence in stress responding. This study provides insights into molecular mechanisms that underlie salt and drought stress tolerance of Phi GSTs and gives evidence for the functional divergence among orthologs in vivo.