文献类型：期刊文献

英文题名：Effects of Cutting, Pruning, and Grafting on the Expression of Age-Related Genes in Larix kaempferi

作者：Zhang, Yao[1] Zang, Qiao-Lu[2] Qi, Li-Wang[2] Han, Su-Ying[1] Li, Wan-Feng[2]

通信作者：Han, SY[1];Li, WF[2]

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry &, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, State Key Lab Tree Genet & Breeding,Key Lab Tree, Beijing, Peoples R China

年份：2020

卷号：11

期号：2

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000519236600018)】；

基金：This research was funded by the National Natural Science Foundation of China (31770714 and 31330017), the Basic Research Fund of Research Institute of Forestry (RIF2014-07), the National Transgenic Major Program (2018ZX08020-003), and the National Key R&D Program of China (2017YFD0601204-2).

语种：英文

外文关键词：larch; vegetative propagation; rejuvenation; gene expression; horticultural technique

摘要：Grafting, cutting, and pruning are important horticultural techniques widely used in the establishment of clonal forestry. After the application of these techniques, some properties of the plants change, however, the underlying molecular mechanisms are still unclear. In our previous study, 27 age-related transcripts were found to be expressed differentially between the juvenile vegetative (1- and 2-year-old) and adult reproductive (25- and 50-year-old) phases of Larix kaempferi. Here, we re-analyzed the 27 age-related transcripts, cloned their full-length cDNA sequences, and measured their responses to grafting, cutting, and pruning. After sequence analysis and cloning, 20 transcription factors were obtained and annotated, most of which were associated with reproductive development, and six (LaAGL2-1, LaAGL2-2, LaAGL2-3, LaSOC1-1, LaAGL11, and LaAP2-2) showed regular expression patterns with L. kaempferi aging. Based on the expression patterns of these transcription factors in L. kaempferi trees subjected to grafting, cutting, and pruning, we concluded that (1) cutting and pruning rejuvenate the plants and change their expression, and the effects of cutting on gene expression are detectable within 14 years, although the cutting seedlings are still maturing during these years; (2) within three months after grafting, the rootstock is more sensitive to grafting than the scion and readily becomes mature with the effect of the scion, while the scion is not readily rejuvenated by the effect of the rootstock; and (3) LaAGL2-2 and LaAGL2-3 are more sensitive to grafting, while LaAP2-2 is impervious to it. These findings not only provide potential molecular markers to assess the state of plants but also aid in studies of the molecular mechanisms of rejuvenation.