文献类型：期刊文献

英文题名：Comparative transcriptomes reveal the disjunction adaptive strategy of Thuja species in East Asia and North America

作者：Chang, Ermei[1] Liu, Xue[2] Chen, Jiahui[3] Sun, Jingyi[1] Yang, Shaowei[1] Liu, Jianfeng[1]

第一作者：常二梅

通信作者：Liu, JF[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, Res Inst Forestry, Beijing 10091, Peoples R China;[2]Chongqing Acad Chinese Mat Med, Chongqing Key Lab Tradit Chinese Med Resource, Endangered Med Breeding Natl Engn Lab, Chongqing 400065, Peoples R China;[3]Chinese Acad Sc, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China

年份：2023

卷号：34

期号：6

起止页码：1963-1974

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;EI(收录号：20233814733333);Scopus(收录号：2-s2.0-85171174676);WOS:【SCI-EXPANDED(收录号:WOS:001066951500001)】；

基金：This work was supported by the National Natural Science Foundation of China (31870664), the 948 Program of National Forestry and Grassland Administration (2013-4-47), and the National Key Research and Development Program of China (2022YFD2200103).

语种：英文

外文关键词：Thuja species; Comparative transcriptomes; East Asia-North America disjunction; Specific gene; Positively selected gene

摘要：The genus Thuja is ideal for investigating the genetic basis of the East Asia-North America disjunction. The biogeographical background of the genus is debatable and an adaptive strategy is lacking. Through the analysis and mining of comparative transcriptomes, species differentiation and positively selected genes (PSGs) were identified to provide information for understanding the environmental adaptation strategies of the genus Thuja. De novo assembly yielded 44,397-74,252 unigenes of the five Thuja species with contig N-50 length ranging from 1,559 to 1,724 bp. Annotations revealed a similar distribution of functional categories among them. Based on the phylogenetic trees constructed using the transcriptome data, T. sutchuenensis was divided first, followed by T. plicata and T. occidentalis. The final differentiation of T. koraiensis and T. standishii formed a clade. Enrichment analysis indicated that the PSGs of the North American Thuja species were involved in plant hormone signal transduction and carbon fixation of photosynthetic organisms pathways. The PSGs of East Asian Thuja were related to phenolic, alkaloid, and terpenoid synthesis, important stress-resistant genes and could increase plant resistance to external environmental stresses. This study discovered numerous aroma synthetic-related PSGs including terpene synthase (TPS) genes and lipid phosphate phosphatase 2 (LPP2), associated with the synthetic aroma of T. sutchuenensis. Physiological indicators, such as the contents of soluble sugars, total chlorophyll, total phenolics, and total flavonoids were determined, which are consistent with the PSGs enrichment pathways associated with adaptive strategies in the five Thuja species. The results of this study provide an important basis for future studies on conservation genetics.