文献类型：期刊文献

英文题名：Genetic Diversity and Population Differentiation of Calanthe tsoongiana, a Rare and Endemic Orchid in China

作者：Qian, Xin[1] Wang, Cai-xia[1] Tian, Min[1]

第一作者：Qian, Xin

通信作者：Tian, M[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China

年份：2013

卷号：14

期号：10

起止页码：20399-20413

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000328620900061)】；

基金：We thank Xiaoling Niu, Jingjing Lian and Xingping Jiang for their assistance in field observation and sample collection. This work was supported by the Key Science and Technology Specific Projects of Zhejiang Province (No. 2012C12909-10) and the Science and Technology Projects of Zhejiang Forestry Department (No. 2012Y02-3).

语种：英文

外文关键词：Calanthe tsoongiana; genetic diversity; ISSR; conservation; Orchidaceae

摘要：Calanthe tsoongiana is a rare terrestrial orchid endemic to China, and this species has experienced severe habitat loss and fragmentation. Inter-simple sequence repeat (ISSR) markers were employed to assess the genetic diversity and differentiation of six populations of C. tsoongiana. Based on 124 discernible fragments yielded by eleven selected primers, high genetic diversity was revealed at the species level; however, genetic diversity at the population level was relatively low. High-level genetic differentiation among populations was detected based on analysis of molecular variance (AMOVA), indicating potential limited gene flow. No significant relationship was observed between genetic and geographic distances among the sampled populations. These results suggested that restricted gene flow might be due to habitat fragmentation and reduced population size as a result of human activities. Based on the findings, several conservation strategies were proposed for the preservation of this threatened species.