文献类型：期刊文献

英文题名：Molecular identification and genetic variation of varieties of Styphnolobium japonicum (Fabaceae) using SRAP markers

作者：Sun, R. X.[1] Zhang, C. H.[1] Zheng, Y. Q.[1] Zong, Y. C.[1] Yu, X. D.[1] Huang, P.[1]

第一作者：Sun, R. X.

通信作者：Zheng, YQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Silviculture, State Key Lab Tree Genet & Breeding,State Forestr, Beijing, Peoples R China

年份：2016

卷号：15

期号：2

外文期刊名：GENETICS AND MOLECULAR RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000384880000062)】；

基金：Research supported by the Special Fund for Public Interest (#201204307) from the State Forestry Administration. We thank Yan He at Beijing Municipal Bureau of Landscape and Forestry and Xiaoman Xie at Shandong Forest Genetic Resources Center for their help in sample collection.

语种：英文

外文关键词：Styphnolobium japonicum; SRAP; Molecular identification; Genetic variation; DNA fingerprints

摘要：Thirty-four Styphnolobium japonicum varieties were analyzed using sequence-related amplified polymorphism (SRAP) markers, to investigate genetic variation and test the effectiveness of SRAP markers in DNA fingerprint establishment. Twelve primer pairs were selected from 120 primer combinations for their reproducibility and high polymorphism. We found a total of 430 amplified fragments, of which 415 fragments were considered polymorphic with an average of 34.58 polymorphic fragments for each primer combination. The percentage of polymorphic fragments was 96.60%, and four primer pairs showed 100% polymorphism. Moreover, simple matched coefficients ranged between 0.68 and 0.89, with an average of 0.785, indicating that the genetic variation among varieties was relatively low. This could be because of the narrow genetic basis of the selected breeding material. Based on the similarity coefficient value of 0.76, the varieties were divided into four major groups. In addition, abundant and clear SRAP fingerprints were obtained and could be used to establish DNA fingerprints. In the DNA fingerprints, each variety had its unique pattern that could be easily distinguished from others. The results demonstrated that 34 varieties of S. japonicum had a relatively narrow genetic variation. Hence, a broadening of the genetic basis of breeding material is necessary. We conclude that establishment of DNA fingerprint is feasible by means of SRAP markers.