文献类型：期刊文献

英文题名：The WUSCHEL-related homeobox 5a (PtoWOX5a) is involved in adventitious root development in poplar

作者：Li, Jianbo[1,2] Zhang, Jin[1] Jia, Huixia[1] Liu, Bobin[3] Sun, Pei[1] Hu, Jianjun[1] Wang, Lijuan[1] Lu, Mengzhu[1]

通信作者：Wang, LJ[1];Lu, MZ[1]|[a0005e27922ec495458cc]王丽娟;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 100091, Peoples R China;[3]Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Basic Forestry & Prote Ctr, Fuzhou 350000, Fujian, Peoples R China

年份：2018

卷号：38

期号：1

起止页码：139-153

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000422839600012)】；

基金：This work was supported by the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2014ZX001-1), the National Natural Science Foundation of China (No. 31270699) and the Joint Research Fund for Overseas Chinese, Hong Kong and Macao Young Scholars (No. 31,428,002).

语种：英文

外文关键词：adventitious root; lateral root; poplar; vegetative propagation; WOX5

摘要：Adventitious rooting is an essential step in vegetative propagation. Currently, the mechanism that regulates adventitious root (AR) development in woody plants is poorly understood. This work demonstrates that Populus tomentosa WUSCHEL-related homeobox 5a (PtoWOX5a) transcription factor is involved in AR development in poplar. PtoWOX5a was specifically expressed in the AR tip and lateral root tip during AR and lateral root regeneration from the stem segment. Phenotypic complementation experiments indicated that the PtoWOX5a can functionally complement AtWOX5 in quiescent center ( QC) cells. Overexpression of PtoWOX5a introduces significant developmental phenotypes in roots and leaves, such as increased AR number, decreased AR length, swollen AR tip and lateral root tip, and decreased leaf number and area. The conserved mechanism of D-type cyclins (CYCD) repression mediated by WOX5 was confirmed in poplar. The co-expression network of PtWOX5a was constructed, which provided clues to reveal the molecular mechanism of PtoWOX5a in AR development in poplar. Taken together, our results suggest that the PtoWOX5a is involved in AR development though cooperating with a series of functional genes.