文献类型：期刊文献

英文题名：Integrated Degradome and Srna Sequencing Revealed miRNA-mRNA Regulatory Networks between the Phloem and Developing Xylem of Poplar

作者：Ding, Changjun[1] Shen, Tengfei[2] Ran, Na[2] Zhang, Heng[2] Pan, Huixin[2] Su, Xiaohua[1] Xu, Meng[2]

第一作者：丁昌俊

通信作者：Su, XH[1];Xu, M[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Key Lab Forest Genet & Biotechnol, Minist Educ, Nanjing 210037, Peoples R China

年份：2022

卷号：23

期号：9

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85128426363);WOS:【SCI-EXPANDED(收录号:WOS:000794369900001)】；

基金：Please add: This work was funded by the National Key Research and Development Program of China [grant number 2021YFD2201205], the National Natural Science Foundation of China (31971679), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (19KJB180001) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：secondary xylem; phloem; small RNAs; degradome; Populus deltoides

摘要：Lignin and cellulose are the most abundant natural organic polymers in nature. MiRNAs are a class of regulatory RNAs discovered in mammals, plants, viruses, and bacteria. Studies have shown that miRNAs play a role in lignin and cellulose biosynthesis by targeting key enzymes. However, the specific miRNAs functioning in the phloem and developing xylem of Populus deltoides are still unknown. In this study, a total of 134 miRNAs were identified via high-throughput small RNA sequencing, including 132 known and two novel miRNAs, six of which were only expressed in the phloem. A total of 58 differentially expressed miRNAs (DEmiRNAs) were identified between the developing xylem and the phloem. Among these miRNAs, 21 were significantly upregulated in the developing xylem in contrast to the phloem and 37 were significantly downregulated. A total of 2431 target genes of 134 miRNAs were obtained via high-throughput degradome sequencing. Most target genes of these miRNAs were transcription factors, including AP2, ARF, bHLH, bZIP, GRAS, GRF, MYB, NAC, TCP, and WRKY genes. Furthermore, 13 and nine miRNAs were involved in lignin and cellulose biosynthesis, respectively, and we validated the miRNAs via qRT-PCR. Our study explores these miRNAs and their regulatory networks in the phloem and developing xylem of P. deltoides and provides new insight into wood formation.