文献类型：期刊文献

英文题名：Molecular mechanism of poisoning to honeybees (Apis mellifera L.) after ingestion of Camellia oleifera nectar

作者：Wang, Yang[1] Liu, Xiaohua[1] Wang, Yiming[1,3] Jin, Mingxia[1] Xie, Guai[1] Zhong, Qiuping[2] Yu, Ailin[1]

第一作者：Wang, Yang

通信作者：Yu, AL[1]

机构：[1]Jiangxi Acad Forestry, Inst Forest Pest Control, Nanchang 330032, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Beijing, Peoples R China;[3]Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China

年份：2024

卷号：27

期号：1

外文期刊名：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY

收录：;Scopus(收录号：2-s2.0-85183999605);WOS:【SCI-EXPANDED(收录号:WOS:001181756300001)】；

基金：This research was supported by the Funding Key R & D Plan-Unveiling and Leading Projects in Jiangxi Province, grant number 20223BBF61012; and Provincial Key R & D Program of Jiangxi, grant Number 20202BBFL63016. The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

语种：英文

外文关键词：Apis mellifera; Cell apoptosis; Camellia oleifera nectar; Dietary restriction; Poisoning

摘要：To explore the molecular mechanism of honeybees (Apis mellifera L.) poisoning death by ingestion of Camellia oleifera nectar, feeding experiments were designed to investigate the poisoning effects. Observed the development process of poisoning symptoms, and collected bees at key experimental nodes for transcriptome sequencing analysis. The results showed that the first poisoning symptom was observed within 0.5 h after ingestion of Camellia oleifera nectar, with death occurring as early as 2 h post-ingestion and all honey bees dying within 4 h. There were four stages in the development of poisoning symptoms: normal flying, crawling (abdominal swelling, inability to fly normally), fluttering (inability to crawl normally), and death. Transcriptome sequencing analyzed the causes of poisoning death indicated that honey bee poisoning occurred with the transduction of dietary restriction signals into cells, resulting in the down-regulated expression gene elements that ensure cell health and inhibit apoptosis, including intracellular NAD+-dependent protein deacetylase sirtuin 1, insulin-like growth factor 1, and heat shock proteins. At the same time, the expression of cysteinyl aspartate specific proteinase 7, which protects programmed cell apoptosis, was also downregulated, resulting in the rapid and disorderly deaths of a large number of cells in individuals in a short period of time, and eventually leading to bees fatality.