文献类型：期刊文献

英文题名：Comparative transcriptome combined with morpho-physiological analyses revealed candidate genes potentially for differential cold tolerance in two contrasting apricot (Prunus armeniacaL.) cultivars

作者：Yu, Dan[1] Liu, Xiaojuan[1] Cui, Yifan[1] Bi, Quanxin[1] Zhao, Yang[1] Li, Dongxing[1] Yu, Haiyan[1] Wang, Libing[1]

第一作者：Yu, Dan

通信作者：Wang, LB[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China

年份：2020

卷号：34

期号：5

起止页码：1205-1217

外文期刊名：TREES-STRUCTURE AND FUNCTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000555621500002)】；

基金：This work was financially supported by the Central Public-Interest Scientific Institution Basal Research Fund (CAFYBB2020ZY003, CAFYBB2019SY005 and CAFYBB2017ZA004). We thank the Apricot Germplasm Resources Orchard (Shanxi, China) for providing the apricot samples and Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac) for editing the English text of a draft of this manuscript.

语种：英文

外文关键词：Apricot; Spring frost; Transcriptome; Differentially expressed genes; RT-qPCR

摘要：Key message We performed a comparative transcriptome combined with morpho-physiological analyses between two differential cold tolerance in two contrasting apricot cultivars. The revealed candidate genes potentially were identified by differential expression analysis. Apricot (Prunus armeniacaL.) is a major (non-wood product) forest tree species that is adversely affected by spring frost. However, the mechanisms regulating gene expression in response to frost under natural conditions remains relatively uncharacterized. In this study, we analyzed the phenotypic, anatomical, physiological and molecular characteristics of pistils from two apricot cultivars that differ regarding cold tolerance under spring frost. A total of 217,142,216 and 218,148,439 clean reads were obtained for the cold-tolerant ('Weixuan 1') and the cold-sensitive ('Longwangmao') sample libraries, respectively. Moreover, 57,689 unigenes (>= 200 bp long) were identified, with an N50 of 2468 bp and a mean length of 1038.08 bp. Furthermore, 1116 up-regulated and 428 down-regulated differentially expressed genes (DEGs) were identified in the two apricot cultivars. Thirteen DEGs related to Ca(2+)signaling, four DEGs involved in MAPK cascades, and thirty DEGs encoding transcription factors were affected by spring frost. The most significant transcriptome changes induced by spring frost were related to carbohydrate metabolism, amino acid metabolism, lipid metabolism, secondary metabolites, plant hormone signal transduction, and terpenoid metabolism. Notably,bHLH75, GDSL esterase/lipase EXL3, GDSL esterase/lipase LTL1, and peroxidase-66 were more highly expressed in 'Weixuan 1' than in 'Longwangmao' under 6:00, suggesting these genes may contribute to the cold tolerance of 'Weixuan 1'. The reliability of our RNA sequencing results was confirmed by quantitative real-time PCR. The data generated in this study may form the basis of future studies on the mechanisms underlying the cold tolerance of apricot under natural field conditions.