文献类型：期刊文献

中文题名：北京西山油松Pinus tabuliformis和樟子松P.sylvestris var.mongolica人工林土壤-林木连续体微生物群落比较

英文题名：A comparison of soil-plant continuum microbial communities in plantations of Pinus tabuliformis and P.sylvestris var.mongolica in West Mountain of Beijing

作者：张从明[1] 满孝武[1] 吴芳[1] 员瑗[1] 边禄森[2]

第一作者：张从明

机构：[1]北京林业大学生态与自然保护学院微生物研究所,北京100083;[2]中国林业科学研究院华北林业实验中心、北京九龙山暖温带森林国家长期科研基地,北京102300

年份：2023

卷号：42

期号：10

起止页码：2041-2062

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

语种：中文

中文关键词：油松;樟子松;微生物群落;土壤-林木连续体;土壤理化性质

外文关键词：Pinus tabuliformis;Pinus sylvestris var.mongolica;microbial community;soil-plant continuum;soil physicochemical properties

分类号：S71

摘要：油松Pinus tabuliformis和樟子松Pinus sylvestris var.mongolica是中国重要造林树种,但具有不同的地理分布。北京西山处于油松生长的适生区,但为樟子松引种的南界,成为探究树种在土壤-林木连续体微生物群落构建驱动作用的理想研究样本。本研究应用高通量测序技术获取了北京西山同一地点的油松和樟子松人工林土壤-林木连续体多个部位的微生物群落组成,探究了树种因素在这2种松树土壤-林木连续体微生物群落构建中发挥的作用。土壤作为林木微生物主要来源具有特殊地位,本研究进一步研究了与土壤微生物关系密切的土壤理化性质。研究结果表明,2种松树土壤-林木连续体微生物群落α多样性差异主要体现在土壤和根际土壤中;β多样性分析表明仅针叶中内生的细菌群落组成在两松树间差异不显著,其他部位的微生物群落在2种松树间均具有一定的组成差异,树种对土壤和根际土壤微生物群落组成方差的解释度最高,而对针叶微生物群落组成方差解释度最低,即树种在土壤和根际土壤微生物群落构建中发挥较大的作用,而在针叶内生微生物群落构建中发挥作用较小。油松比樟子松相同部位细菌群落具有更加复杂的网络结构,细菌生态网络属性在2种松树间具有清晰差异,但真菌没有。油松和樟子松土壤-林木连续体中含有丰富的微生物类群,以相对多度大于5%的纲作为优势纲,共有7个细菌纲和7个真菌纲组成了2种松树各个部位的优势纲,优势细菌纲为:Alphaproteobacteria、Betaproteobacteria、Actinobacteria、Thermoleophilia、Gammaproteobacteria、Bacilli和Clostridia,优势真菌纲为Agaricomycetes、Leotiomycetes、Sordariomycetes、Eurotiomycetes、Dothideomycetes、Mortierellomycetes和Pezizomycetes。土壤理化性质是影响2种松树人工林土壤中微生物多样性差异和组成的重要因素,其中细菌和真菌群落组成均受到有效磷(availablephosphorus,AP)、pH和速效钾(availablekalium,AK)的影响,此外,细菌群落的组成还显著与碱解氮(alkali-hydrolyzed nitrogen,AHN)相关。研究结果揭示了树种差异在华北地区油松和樟子松土壤-林木连续体微生物群落构建中的作用、松树与微生物的互作,也为华北地区松树造林提供了林下微生物数据。
Pinus tabuliformis and Pinus sylvestris var.mongolica are important afforestation species with different geographical distributions in China.The West Mountain of Beijing is the suitable area for the growth of Pinus tabuliformis,but is the southern extremity of distribution of P.sylvestris var.mongolica,therefore it is an ideal research site to explore the driving role of tree species in the construction of microbial community in soil-plant continuum.In this study,high-throughput sequencing technology was used to obtain the composition of microbial communities in several parts of the soil-plant continuum of plantations of P.tabuliformis and P.sylvestris var.mongolica in West Mountain of Beijing.The role of species factors in the construction of soil-plant continuum microbial community of these two pine trees was explored,and the physicochemical properties of soil closely related to soil microorganisms were further studied.The results showed that the difference of alpha diversity of microbial community in soil-plant continuum between the two pine was mainly reflected in bulk soil and rhizosphere soil.The beta diversity analysis showed that the bacterial community composition in needle was not significant difference between the two pine,while the microbial community composition in other parts of tree was different between the two pine.Tree species had the highest explanation for the variance of soil and rhizosphere microbial community composition,while the lowest explanation for the variance of needle microbial community composition,in other words,tree species played a larger role in the construction of soil and rhizosphere microbial community,but a smaller role in the construction of needle endophytic microbial community.The network structure of bacterial communities in the same part of tree was more complex in P.tabuliformis than in P.sylvestris var.mongolica,but the fungal network had less differences between the two pines.Abundant microbial groups in the tissues and soil of P.tabuliformis and P.sylvestris var.mongolica were found.At class level,the relative abundance greater than 5%was considered as dominant class.Seven bacterial classes,Alphaproteobacteria,Betaproteobacteria,Actinobacteria,Thermoleophilia,Gammaproteobacteria,Bacilli,and Clostridia,and seven fungal classes,Agaricomycetes,Leotiomycetes,Sordariomycetes,Eurotiomycetes,Dothideomycetes,Mortierellomycetes,and Pezizomycetes,were recognized.Significant differences in soil physicochemical properties,e.g.available phosphorus(AP),pH and available kalium(AK),were found between the plantations of P.tabuliformis and P.sylvestris var.mongolica,and these properties effect soil microbial diversity and composition between the two pines.In addition,alkali-hydrolyzed nitrogen(AHN)was also significantly correlated with the composition of the bacterial community.The results demonstrate the interactions between pines and microorganisms and the mechanism of microbial community construction,and provide reference data on microbial community in pine plantations in northern China.