文献类型：期刊文献

英文题名：Molecular evolution and functional characterization of chitinase gene family in Populus trichocarpa

作者：Zhang, Yuan-Jie[1] Ren, Lin-Ling[2] Lin, Xiao-Yang[3] Han, Xue-Min[4] Wang, Wei[1] Yang, Zhi-Ling[5]

第一作者：Zhang, Yuan-Jie

通信作者：Yang, ZL[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China;[2]Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China;[3]Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China;[4]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[5]Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Forest Ecol, Mengla 666303, Peoples R China

年份：2022

卷号：822

外文期刊名：GENE

收录：;Scopus(收录号：2-s2.0-85126113303);WOS:【SCI-EXPANDED(收录号:WOS:000776237900008)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (NSFC 31400574) .

语种：英文

外文关键词：Chitinase; Functional divergence; Poplar; Stress response; Tandem duplication

摘要：Chitinases, the chitin-degrading enzymes, have been shown to play important role in defense against the chitincontaining fungal pathogens. In this study, we identified 48 chitinase-coding genes from the woody model plant Populus trichocarpa. Based on phylogenetic analysis, the Populus chitinases were classified into seven groups. Different gene structures and protein domain architectures were found among the seven Populus chitinase groups. Selection pressure analysis indicated that all the seven groups are under purifying selection. Phylogenetic analysis combined with chromosome location analysis showed that Populus chitinase gene family mainly expanded through tandem duplication. The Populus chitinase gene family underwent marked expression divergence and is inducibly expressed in response to treatments, such as chitosan, chitin, salicylic acid and methyl jasmonate. Protein enzymatic activity analysis showed that Populus chitinases had activity towards both chitin and chitosan. By integrating sequence characteristic, phylogenetic, selection pressure, gene expression and protein activity analysis, this study shed light on the evolution and function of chitinase family in poplar.