文献类型：期刊文献

英文题名：Hybrid Genome Assembly and Gene Repertoire of the Root Endophyte Clitopilus hobsonii QYL-10 (Entolomataceae, Agaricales, Basidiomycetes)

作者：Peng, Long[1,2] Shan, Xiaoliang[1,2,3] Wang, Yuchen[1,2,3] Martin, Francis[4] Vilgalys, Rytas[5] Yuan, Zhilin[1,2]

第一作者：彭龙

通信作者：Yuan, ZL[1];Yuan, ZL[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[3]Nanjing Forestry Univ, Nanjing 210037, Peoples R China;[4]Univ Lorraine, INRAe, UMR Interact Arbres Microorg 1136, INRAe Grand Est Nancy, F-54280 Champenoux, France;[5]Duke Univ, Dept Biol, Box 90338, Durham, NC 27708 USA

年份：2021

卷号：34

期号：6

起止页码：711-714

外文期刊名：MOLECULAR PLANT-MICROBE INTERACTIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000709760300014)】；

基金：This work was funded by Fundamental Research Funds for the Central Non-profit Research of Chinese Academy of Forestry (CAFYBB2020QB002) and National Natural Science Foundation of China (numbers 31901290 and 31722014).

语种：英文

外文关键词：fungal endophytes; genomics; long-read assembly; root-fungal symbiosis; saprotrophy-biotrophy transition

摘要：Clitopilus hobsonii (Entolomataceae, Agaricales, Basidiomycetes) is a common soil saprotroph. There is also evidence that C. hobsonii can act as a root endophyte benefitting tree growth. Here, we report the genome assembly of C. hobsonii QYL-10, isolated from ectomycorrhizal root tips of Quercus lyrata. The genome size is 36.93 Mb, consisting of 13 contigs (N-50 = 3.3 Mb) with 49.2% GC content. Of them, 10 contigs approached the length of intact chromosomes, and three had telomeres at one end only. BUSCO analysis reported a completeness score of 98.4%, using Basidiomycota_odb10 lineage data. Combining ab-initio, RNA-seq data, and homology-based predictions, we identified 12,710 protein-coding genes. Approximately, 1.43 Mb of transposable elements (3.88% of the assembly), 36 secondary metabolite biosynthetic gene clusters, and 361 genes encoding putative carbohydrate-active enzymes were identified. This genomic resource will allow functional studies aimed to characterize the symbiotic interactions between C. hobsonii and its host trees and will also provide a valuable foundation for further research on comparative genomics of the Entolomataceae.