文献类型：期刊文献

英文题名：Acidobacteria Community Responses to Nitrogen Dose and Form in Chinese Fir Plantations in Southern China

作者：Liu, Caixia[1,2,3] Dong, Yuhong[1,2,3] Hou, Lingyu[1,2,3] Deng, Nan[1,2,3] Jiao, Ruzhen[1,2,3]

第一作者：Liu, Caixia

通信作者：Jiao, RZ[1];Jiao, RZ[2];Jiao, RZ[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[2]State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Key Lab Tree Breeding & Cultivat State Forestry A, Beijing 100091, Peoples R China

年份：2017

卷号：74

期号：3

起止页码：396-403

外文期刊名：CURRENT MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000395129800013)】；

基金：This study was supported in part by [1] the National Key Research Program of China (No. 2016YFD0600302) and [2] Research Institute of Forestry, the Chinese Academy of Forestry (No. CAFYBB2012026).

语种：英文

外文关键词：Acidobacteria; Subgroup; Ammonium; Nitrate; N dose; Forest soil

摘要：Acidobacteria is a new bacterial group, identified by molecular research, which is widely distributed and has specific ecological functions in forest soil. In this study, we investigated Acidobacteria response to N input, and the effects were related to N form and dose. The experimental design included two N forms (NH4 (+)-N and NO3 (-)-N) and five levels of N deposition (0, 20, 40, 60, 80 kg N ha(-1)) for 2 years. Research into the Acidobacteria community was conducted using 16Sr RNA gene-based high-throughput pyrosequencing methods. Acidobacteria OTUs and N had a negative relationship in 0-60 kg ha(-1) year(-1); however, at N doses beyond a certain size, nitrogen might promote an increase in Acidobacteria OTUs. The Acidobacteria relative abundance under NH4 (+)-N treatment was higher than under NO3 (-)-N treatment. Acidobacteria relative abundance decreased with increasing of NH4 (+)-N dose, but increased with increasing NO3 (-)-N dose. Overall, 13 different Acidobacteria subgroups were identified, with Gp1, Gp2, and Gp3 being dominant. Significant differences in Acidobacteria distribution were primarily caused by N input and pH value. The environmental factors of N were all negatively related to Acidobacteria distribution in low N dose treatments (0-20 kg ha(-1) year(-1)), but were positively related in response to N dose treatments (40-80 kg ha(-1) year(-1)).