文献类型：期刊文献

英文题名：Microwave-assisted deep eutectic solvents (DES) pretreatment of control and transgenic poplars for boosting the lignin valorization and cellulose bioconversion

作者：Ma, Cheng-Ye[1] Gao, Xue[1] Peng, Xiao-Peng[2] Gao, Yu-Fei[1] Liu, Jing[1,3] Wen, Jia-Long[1,4] Yuan, Tong-Qi[1,4]

第一作者：Ma, Cheng-Ye

通信作者：Wen, JL[1]

机构：[1]Beijing Forestry Univ, Beijing Key Lab Lignocellulos Chem, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Washington Univ, Energy Environm & Chem Engn, St Louis, MO 63130 USA;[4]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, 35 Tsinghua East Rd, Beijing 100083, Peoples R China

年份：2021

卷号：164

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20211310140727);Scopus(收录号：2-s2.0-85103088821);WOS:【SCI-EXPANDED(收录号:WOS:000636113000001)】；

基金：This work was supported by the National Natural Science Foundation of China (31872698; 32071854), Beijing Forestry University Outstanding Young Talent Cultivation Project (2019JQ03006) and National Key Research and Development Program of China (2019YFB1503801), the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2018MA006).

语种：英文

外文关键词：Transgenic poplars; DES pretreatment; Lignin characterization; Antioxidant activity; Enzymatic saccharification

摘要：Reducing the ?biomass recalcitrance? and simultaneously improving the efficiency of deconstruction and conversion is vitally important in a biorefinery process. Herein, a microwave-assisted deep eutectic solvents (DES) pretreatment was developed to deconstruct the control and transgenic (C3H, HCT and C3H + HCT co-inhibition) poplars, and improve the lignin extractability and valorization. NMR and GPC results showed that native lignin (Double Enzymatic Lignin, DEL) in different poplars exhibited slightly different structural characteristics, affecting the subsequent lignin extractability and value-added applications. After two kinds of DES (ethylene glycol-based and glycerol-based DES) pretreatments, the yield of DES lignin was obviously increased to 45.38?53.09 % and 46.38?54.30 % in transgenic poplar woods as compared to control poplar wood (37.25 and 38.86 %). In addition, molecular weight, and abundances of different linkages (13-O-4, 13-13, 13-5, etc.) in the DES lignin fractions were significantly decreased, suggesting the microwave-assisted DES pretreatment has a strong deconstruction ability towards to poplar wood. Moreover, the rapid and efficient pretreatment facilitated the isolation of the lignin fractions from transgenic poplars, and the regenerated DES lignin fractions exhibited excellent antioxidant properties, which will lay the foundation for the lignin valorization. Furthermore, the enzymatic saccharification ratios of the DES pretreated substrates were significantly increased to 87.36?96.55 % in control and transgenic poplar woods, indicating that microwave-assisted DES pretreatment indeed boosted cellulose bioconversion. In short, the combination of genetic modification and DES pretreatment can significantly reduce the ?biomass recalcitrance? and promote the biomass valorization.