文献类型：期刊文献

英文题名：Identification of putative genes for polyphenol biosynthesis in olive fruits and leaves using full-length transcriptome sequencing

作者：Rao Guodong[1] Zhang Jianguo[1,2,3] Liu Xiaoxia[1] Luo Ying[1]

第一作者：饶国栋

通信作者：Rao, GD[1];Zhang, JG[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2019

卷号：300

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20193107246709);Scopus(收录号：2-s2.0-85069740601);WOS:【SCI-EXPANDED(收录号:WOS:000483990100034)】；

基金：This work was supported by The National Key R&D Program of China (2018YFD1000603-3) and Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2018QB001). We thank Suzhou Bionovogene (http://www.bionovogene.com) for its help in data analysis. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

语种：英文

外文关键词：Olea europaea; Polyphenol; Oleuropein; Hydroxytyrosol; Full-length transcripts; Alternative splicing

摘要：Olive (Olea europaea) is a rich source of valuable bioactive polyphenols, which has attracted widespread interest. In this study, we combined targeted metabolome, Pacbio ISOseq transcriptome, and Illumina RNA-seq transcriptome to investigate the association between polyphenols and gene expression in the developing olive fruits and leaves. A total of 12 main polyphenols were measured, and 122 transcripts of 17 gene families, 101 transcripts of 9 gene families, and 106 transcripts of 6 gene families that encode for enzymes involved in flavonoid, oleuropein, and hydroxytyrosol biosynthesis were separately identified. Additionally, 232 alternative splicing events of 18 genes related to polyphenol synthesis were analyzed. This is the first time that the third generations of full-length transcriptome technology were used to study the gene expression pattern of olive fruits and leaves. The results of transcriptome combined with targeted metabolome can help us better understand the polyphenol biosynthesis pathways in the olive.