文献类型：期刊文献

英文题名：De Novo Transcriptome Analysis of Dalbergia odorifera T. Chen (Fabaceae) and Transferability of SSR Markers Developed from the Transcriptome

作者：Liu, Fu-Mei[1,2] Hong, Zhou[3] Yang, Zeng-Jiang[3] Zhang, Ning-Nan[3] Liu, Xiao-Jin[3] Xu, Da-Ping[3]

第一作者：Liu, Fu-Mei

通信作者：Hong, Z[1]

机构：[1]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Heilongjiang, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Trop Forestry, Pingxiang 532600, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2019

卷号：10

期号：2

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000460744000022)】；

基金：This research was funded by the National Natural Science Foundation of China (31500537), the Natural Science Foundation of Guangxi Province (2016GXNSFBA380089), and the Central Non-Profit Research Institution of Chinese Academy of Forestry (Grant No. CAFYBB2017ZX001-4).

语种：英文

外文关键词：Dalbergia odorifera T; Chen; Dalbergia tonkinensis Prain; Dalbergia cochinchinensis Pierre ex Laness; molecular marker; cross-amplification; rosewood

摘要：Dalbergia odorifera T. Chen (Fabaceae), indigenous to Hainan Island, is a precious rosewood (Hainan hualimu) in China. However, only limited genomic information is available which has resulted in a lack of molecular markers, limiting the development and utilization of the germplasm resources. In this study, we aim to enrich genomic information of D. odorifera, and develop a series of transferable simple sequence repeat (SSR) markers for Dalbergia species. Therefore, we performed transcriptome sequencing for D. odorifera by pooling leaf tissues from three trees. A dataset of 138,516,418 reads was identified and assembled into 115,292 unigenes. Moreover, 35,774 simple sequence repeats (SSRs) were identified as potential SSR markers. A set of 19 SSR markers was successfully transferred across species of Dalbergia odorifera T. Chen, Dalbergia tonkinensis Prain, and Dalbergia cochinchinensis Pierre ex Laness. In total, 112 alleles (3-13 alleles/locus) were presented among 60 Dalbergia trees, and polymorphic information content ranged from 0.38 to 0.75. The mean observed and mean expected heterozygosity was 0.34 and 0.40 in D. odorifera, 0.27 and 0.32 in D. tonkinensis, and 0.29 and 0.33 in D. cochinchinensis, respectively. The cluster analysis classified these 60 trees into three major groups according to the three Dalbergia species based on the genetic similarity coefficients, indicating these newly developed transferable markers can be used to explore the relationships among Dalbergia species and assist genetic research. All these unigenes and SSR markers will be useful for breeding programs in the future.