文献类型：期刊文献

英文题名：Integrated metabolome and transcriptome analysis unveils novel pathway involved in the fruit coloration of Nitraria tangutorum Bobr.

作者：Zhang, Huilong[1,2] Hu, Aishuang[1,2,3] Wu, Haiwen[1,2] Zhu, Jianfeng[1,2] Zhang, Jingbo[4] Cheng, Tielong[5] Shabala, Sergey[6,7] Zhang, Huaxin[1,2] Yang, Xiuyan[1,2]

第一作者：Zhang, Huilong

通信作者：Yang, XY[1];Yang, XY[2]

机构：[1]Chinese Acad Forestry, Inst Ecol Protect & Restorat, Beijing 10091, Peoples R China;[2]Chinese Acad Forestry Yellow River Delta, Comprehens Expt Ctr, Dongying 257000, Peoples R China;[3]Hebei Acad Agr & Forestry Sci, Inst Coastal Agr, Tangshan 063299, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou 015200, Peoples R China;[5]Nanjing Forestry Univ, Minist Educ, Coinnovat Ctr Sustainable Forestry Southern China, Key Lab Forestry Genet & Biotechnol, Nanjing 210037, Peoples R China;[6]Univ Tasmania, Tasmanian Inst Agr, Hobart, Tas 7001, Australia;[7]Foshan Univ, Int Res Ctr Environm Membrane Biol, Foshan 528000, Peoples R China

年份：2023

卷号：23

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85147170433);WOS:【SCI-EXPANDED(收录号:WOS:000922601700006)】；

基金：This work was supported by the Fundamental Research Funds of CAF (project no. CAFYBB2020MA007 and CAFYBB2021MB004), the National Natural Science Foundation of China Grant (31870663).The funders played no role in designing the study, collecting and analyzing the data, writing the manuscript, or deciding the publish.

语种：英文

外文关键词：Nitraria tangutorum; Anthocyanin; Multi-omics; Regulatory networks; Halophyte

摘要：BackgroundThe desert shrub Nitraria tangutorum Bobr. is important for its resistance to salt and alkali in Northwest China. It is an ecologically important species in this region and provides edible and medicinal berries. This study showed a mutant of N. tangutorum (named Jincan, JC) that has a strong yellow pericarp vs red in a wild type (represented by NT).ResultsIn this study, the secondary metabolic and molecular mechanisms responsible for Nitraria fruit coloration were investigated using LC-MS-based widely targeted metabolomics and transcriptomics data. As a result of our study, 122 and 104 flavonoid metabolites were differentially expressed throughout the mature and transition stages between JC and NT, respectively. Furthermore, two cyanidin derivatives (cyanidin 3-O-glucoside and cyanidin-3-O-(2''-O-glucosyl) glucoside) and one pelargonidin derivative (pelargonidin-3-O-glucoside) were identified only in the NT phenotype. The functional genes F3H (flavanone 3-hydroxylase), F3'H (flavonoid 3 '-hydroxylase) and UFGT (flavonoid 3-O-glucosyltransferase) and the transcription factors MYB, bHLH, NAC and bZIP were significantly downregulated in JC. Meanwhile, the activity of UFGT was extremely low in both periods of JC, with a five-fold higher enzymatic activity of UFGT in RT than in YT. In summary, due to the lack of catalysis of UGFT, yellow fruit of JC could not accumulate sufficient cyanidin and pelargonidin derivatives during fruit ripening.ConclusionTaken together, our data provide insights into the mechanism for the regulation of anthocyanin synthesis and N. tangutorum fruit coloration and provide a theoretical basis to develop new strategies for developing bioactive compounds from N. tangutorum fruits.