详细信息
Sucrose induces rapid activation of CfSAPK, a mitogen-activated protein kinase, in Cephalostachyum fuchsianum Gamble cells ( SCI-EXPANDED收录) 被引量:3
文献类型:期刊文献
英文题名:Sucrose induces rapid activation of CfSAPK, a mitogen-activated protein kinase, in Cephalostachyum fuchsianum Gamble cells
作者:Li, Lubin[2] Li, Yuan[1] Zhang, Lu[1] Xu, Chunhui[1] Su, Tongbing[1] Ren, Dongtao[1] Yang, Hailian[1]
第一作者:李潞滨
通信作者:Yang, HL[1]
机构:[1]China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China
年份:2012
卷号:35
期号:8
起止页码:1428-1439
外文期刊名:PLANT CELL AND ENVIRONMENT
收录:;Scopus(收录号:2-s2.0-84863519734);WOS:【SCI-EXPANDED(收录号:WOS:000306006000007)】;
基金:This work was supported by grants from the State Basic Research Program (2012CB114200 to D. R.), the National Natural Science Foundation of China (30870220 and 31125006 to D. R., 30771124 to H.Y., J1103520 to L.Z. and C. X., 31000127 to Y.L.), the Zhejiang Municipal Science and Technology Project (2009C12097 to L.L.).
语种:英文
外文关键词:sucrose signalling; suspension cells
摘要:Sucrose was recently demonstrated to function as a molecular signal. However, sucrose-specific sensing and signalling pathways remain largely undefined. Here, we show that Cephalostachyum fuchsianum sucrose-activated protein kinase (CfSAPK) is transiently and specifically activated by sucrose in C. fuchsianum Gamble suspension cells. The result suggested that CfSAPK participates in a sucrose-signalling pathway. CfSAPK was partially purified from sucrose-treated cells and further analysed. Kinase activity assays revealed that CfSAPK preferentially used myelin basic protein (MBP) as substrate in vitro and strongly phosphorylate MBP threonine residue(s) and weakly phosphorylated MBP serine residue(s). Of the divalent cations tested, Mg2+ was required for CfSAPK activation. Phosphatase treatment of CfSAPK abolished its kinase activity, indicating that phosphorylation is required for CfSAPK activation. Seven internal tryptic peptides identified from CfSAPK matched mitogen-activated protein kinases (MAPKs) in plants. CfSAPK cDNA was cloned using RT-PCR and rapid amplification of cDNA ends (RACE). CfSAPK cDNA encodes a 382-amino acid protein with a calculated molecular mass of 43 466.9 Da. The CfSAPK protein contains all 11 conserved kinase subdomains found in other Ser/Thr kinases. The amino acids sequence of CfSAPK is highly homologous to group A MAPKs in monocotyledon plants.
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