文献类型：期刊文献

英文题名：Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (Robinia pseudoacacia L.) without Castration

作者：Sun, Yuhan[1] Hu, Ruiyang[2] Dong, Li[1] Li, Xiuyu[1] Zhang, Zijie[1] Guo, Qi[1,3] Cao, Sen[1] Li, Jiankang[1] Han, Peiyao[1] Han, Chao[1] Uddin, Saleem[1] Long, Cui[1] Fan, Yingming[1] Li, Yun[1]

第一作者：Sun, Yuhan

通信作者：Li, Y[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Engn Technol Res Ctr Black Locust Natl Forestry &, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Forestry North China, Natl Permanent Sci Res Base Warm Temperate Zone F, Beijing 102300, Peoples R China;[3]Henan Univ Sci & Technol, Coll Agr, Luoyang 471023, Peoples R China

年份：2021

卷号：12

期号：10

外文期刊名：FORESTS

收录：;EI(收录号：20214411102695);Scopus(收录号：2-s2.0-85118200258);WOS:【SCI-EXPANDED(收录号:WOS:000715235500001)】；

基金：FundingThis research was funded by the Fundamental Research Funds for the Central Universities (2015ZCQ-SW-03), National Key R&D Program of China (2017YFD0600503), and National Nature Science Foundation of China (31570677).

语种：英文

外文关键词：

Robinia pseudoacacia L.

; hybridization breeding; artificial controlled pollination; without castration; pollen competition; outcrossing rate; honeybee

摘要：(1) Background: Considering the serious damage caused by castration and the extremely high outcrossing rate in nature, we hypothesized that artificial controlled pollination of black locust without castration could be conducted for hybridization breeding. (2) Methods: The study conducted controlled pollination on 20 mating combinations of black locust without castration using a single or mixed male parent. Offspring of different developmental stages and the leaves of parents were collected to extract DNA and perform paternity analysis using SSR molecular markers. (3) Results: The contribution rate of each male parent differed according to developmental stage after pollination using different pollens mixed in equal proportions. There were significant correlations between the genetic similarity between each male parent and female parent and contribution rate of each male parent at three different developmental stages after pollination. (4) Conclusions: The composition of offspring pollen donors showed no bias toward selfing or outcrossing when artificially pollinated without castration. Hybrid breeding of black locust by artificially controlled pollination without castration may not be feasible, given that our manual method resulted in a large number of abortive and abnormal offspring. Introduction of honeybees in a limited space to conduct controlled pollination of black locust for hybrid breeding may be feasible.