文献类型：期刊文献

英文题名：A mitochondria-target probe for OCl- "naked eye" detection and its imaging in living cell

作者：Zhong, Xiuli[1] Zhou, Lin[1] Jin, Can[2] Wang, Bingxiang[1] Jiang, Yuliang[1] Shen, Jian[1]

第一作者：Zhong, Xiuli

通信作者：Jiang, YL[1];Shen, J[1]

机构：[1]Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Sch Chem & Mat Sci, Jiangsu Key Lab Biofunct Mat, Nanjing 210023, Jiangsu, Peoples R China;[2]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Key Lab Biomass Energy & Mat Jiangsu Prov, Nanjing 210042, Jiangsu, Peoples R China

年份：2019

卷号：202

起止页码：369-374

外文期刊名：TALANTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000472693700048)】；

基金：This work was supported the China Postdoctoral Fund Surface Project (2017M610335), Natural Science Foundation of Jiangsu Province (BK20181123), Fundamental Research Funds of Jiangsu Key Lab of Biomass Energy and Material (JSBEM-S-201806).

语种：英文

外文关键词：Hypochloric acid; Mitochondrion; Fluorescent probe; Naked eye; Bioimaging

摘要：Hypochloric acid (HOCl) plays vital roles in cell signaling and homeostasis which involved in many related diseases. Therefore, the accurate detected level of Cr for discussing the complex contributions of OCl- to human health is of great significance. In this study, a novel mitochondrion-targeting fluorescent probe DMI bearing a carbon-carbon double bond as an OCl--responsive site has been developed. Probe DMI exhibited specific fluorescence response toward OCl- with fast response (within 4 s) and high sensitivity (detection limit is 0.05 mu M), where the obvious color changes could be observed by the naked eye. More importantly, DMI could be applied in the bioimaging of OCl- in living A549 cells successfully benefited from its good sensing properties and low toxicity. Fluorescence co-localization test was further carried out and confirmed mitochondria-targetable ability of DMI which can be used for investigating physiological function of HOCl at organelle levels.