文献类型：期刊文献

英文题名：Transcriptome analysis reveals the developmental dynamic of stem in poplar

作者：Zhang, Yanhui[1] Chen, Song[2] Chen, Su[2] Yue, Jing[1] Liu, Yingli[1] Li, Quanzi[1]

通信作者：Liu, YL[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China

年份：2024

卷号：222

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20243516929756);Scopus(收录号：2-s2.0-85201780652);WOS:【SCI-EXPANDED(收录号:WOS:001301650000001)】；

基金：This work was supported by the National Key Research and Devel-opment Program of China (2023YFD2200204) .

语种：英文

外文关键词：poplar stem; Primary growth; Secondary growth; WGCNA; Wood formation

摘要：Woody plants undergo primary growth and secondary growth, which produces biomass for biofuel. However, our insight on the regulation of gene expressions and the dynamic changes of the biological processes that underlie the two types of growth remains incomplete. In this study, we conducted anatomic observations on the stem development from 2nd to 7th internode in Populus alba x Populus glandulosa stem development and examined the gene expression patterns across these internodes by transcriptome profiling. RNA-seq analysis showed that the comparisons of 6th internode vs 5th internode and of 7th internode vs 6th internode identified more differentially expressed gene (DEG), indicating extensive transcriptional reprogramming during these transitions. Enrichment analyses of DEGs unveiled distinct biological processes associated with various internodes, indicating the initiation of secondary growth. WGCNA (Weighted Gene Co-expression Network Analysis) identified the modules that were highly correlated with specific internodes, and consistent with trend analysis identified secondary cell wall (SCW) clusters as the largest clusters/modules. SCW formation related genes were fully activated at the 7th internode, and xylan biosynthetic enzyme genes were expressed earlier than lignin and cellulose biosynthetic enzyme genes. Regulatory network analysis unveiled 716 potential (transcription factor) TF-gene interactions governing cellulose, xylan, and lignin biosynthesis, emphasizing the pivotal role of TFs like MYB and SND1 in SCW formation. Overall, our findings elucidate the regulatory dynamics that drive the transition from primary to secondary growth, providing valuable insights into wood formation mechanisms.