文献类型：期刊文献

中文题名：山茶杂交后代花青苷变异特征分析

英文题名：Evaluation of salt tolerance and screening of salt-tolerant SNP markers in F1 generation derived from wild Rosa rugosa×R.rugosa‘Alba Plena’

作者：张莹[1] 杨美英[1,2] 李建宾[2] 李辛雷[1] 刘信凯[3] 钟乃盛[3] 黎艳玲[3]

第一作者：张莹

机构：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400;[2]云南农业大学农学与生物技术学院,云南昆明650201;[3]肇庆棕榈谷花园有限公司,广东肇庆526112

年份：2025

卷号：42

期号：5

起止页码：994-1002

中文期刊名：浙江农林大学学报

外文期刊名：Journal of Zhejiang A&F University

收录：;北大核心:【北大核心2023】；

基金：浙江省农业(花卉新品种选育)新品种选育重大科技专项(2021C02071-2)。

语种：中文

中文关键词：杜鹃红山茶;越南抱茎茶;杂交后代;花青苷;变异

外文关键词：Camellia azalea;C.amplexicaulis;hybrids;anthocyanins;variation

分类号：S68

摘要：【目的】研究杜鹃红山茶Camellia azalea、越南抱茎茶C.amplexicaulis及其杂交后代花瓣中主要花青苷的成分,明确其花色形成的物质基础,揭示主要花青苷成分与质量分数的变异特征,为山茶花种质创新及高效利用提供科学依据。【方法】应用CIE L*a*b*表色系法进行花色测定,利用高效液相色谱-二极管阵列检测(HPLC-DAD)和超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)联用技术定性定量分析花瓣中的花青苷成分与质量分数,运用多元线性回归方法明确花色与花青苷之间的关系。【结果】在亲本及其18个杂交后代中共检测到16种花青苷。杂交后代的花青苷总量基本介于双亲之间;花青苷矢车菊素-3-O-[6-O-(E)-p-香豆酰]-葡萄糖苷(Cy3GEpC)、矢车菊素-3-O-[2-O-木糖基-6-O-(E)-p-香豆酰]-葡萄糖苷(Cy3GEpCX)和矢车菊素-3-O-[6-O-(E)-p-香豆酰]-半乳糖苷(Cy3GaEpC)变异较大,其质量分数大部分高于亲本,其他花青苷的质量分数基本介于双亲之间。【结论】杂交后代中含半乳糖苷和2-O-木糖基的花青苷成分主要来源于杜鹃红山茶,含葡萄糖苷及不含2-O-木糖基的花青苷成分来源于双亲。矢车菊素-3-O-[2-O-木糖基-6-O-(E)-p-香豆酰]-半乳糖苷(Cy3GaEpCX)和矢车菊素-3-O-半乳糖苷(Cy3Ga)是影响杜鹃红山茶与越南抱茎茶杂交后代花色的主要花青苷,与花色变异显著相关。
[Objective]The object of this study is to determine anthocyanin components in petals from Camellia azalea,C.amplexicaulis and their hybrids.The material basis of their color formation is clarified and the variability characteristics of main anthocyanin components and contents are revealed,which will provide a basis for the germplasm innovation and their efficient utilization.[Method]Flower colors of C.azalea,C.amplexicaulis and their hybrids were measured by CIE L*a*b*scale.Anthocyanin components and contents of C.azalea,C.amplexicaulis and their hybrids were measured by high-performance liquid chromatography coupled with diode array detection(HPLC-DAD)and ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).The relationship between the variation of flower colors and anthocyanin components was explored by multiple liner regression analyses.[Result]16 anthocyanin components were detected in C.azalea,C.amplexicaulis and 18 hybrids.The total anthocyanin in hybrids was between the parents.The variation of Cy3GaEpC、Cy3GEpCX and Cy3GEpC in hybrids was large,and most of their contents were higher than that of parents,and the rest components were basically between parents.[Conclusion]Anthocyanins with galactoside in hybrids entirely came from C.azalea,and anthocyanins with 2-O-xylosyl mainly came from C.azalea.Anthocyanins with glucoside and without 2-O-xylosyl came from C.azalea and C.amplexicaulis.Cy3GaEpCX and Cy3Ga were the main anthocyanins affecting the hybrids flower color,and had significant correlation with color variation.