文献类型：期刊文献

英文题名：Up-Converting Nanoparticle-Based Immunochromatographic Strip for Multi-Residue Detection of Three Organophosphorus Pesticides in Food

作者：Zou, Rubing[1] Chang, Yunyun[1] Zhang, Tianyun[1] Si, Fangfang[1] Liu, Ying[1] Zhao, Ying[1] Liu, Yihua[1,2] Zhang, Mingzhou[3] Yu, Xiaoping[3] Qiao, Xusheng[4] Zhu, Guonian[1] Guo, Yirong[1]

第一作者：Zou, Rubing

通信作者：Liu, YH[1];Guo, YR[1];Liu, YH[2]

机构：[1]Zhejiang Univ, Minist Agr, Inst Pesticide & Environm Toxicol, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Zhejiang, Peoples R China;[3]China Jiliang Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China;[4]Zhejiang Univ, Dept Mat Sci & Engn, Hangzhou, Zhejiang, Peoples R China

年份：2019

卷号：7

期号：FEB

外文期刊名：FRONTIERS IN CHEMISTRY

收录：;Scopus(收录号：2-s2.0-85068531097);WOS:【SCI-EXPANDED(收录号:WOS:000458034200001)】；

基金：This study was financially supported by the National Key R&D Program of China (2017YFF0210200 & 2016YFD0201302) and the National Natural Science Foundation of China (31401768).

语种：英文

外文关键词：organophosphorus pesticide; up-conversion nanoparticles; lateral flow strip; immunoassay; multi-residue detection

摘要：Organophosphorus (OP) pesticides are widely used to control pests because of their high activity. This study described a rapid and sensitive lateral flow immunochromatographic (LFIC) assay based on up-converting nanoparticles (UCNPs) for multi-residue detection of three OP pesticides. The developed assay integrated novel fluorescent material UCNPs labeled with a broad-specific monoclonal antibody. Based on the competitive platform by immobilized antigen in the test zone, the optimized UCNPs-LFIC assay enabled sensitive detection for parathion, parathion-methyl, and fenitrothion with IC50 of 3.44, 3.98, and 12.49 ng/mL (R-2 >= 0.9776) within 40 min. The detectable ability ranged from 0.98 to 250 ng/mL. There was no cross-reactivity with fenthion, phoxim, isocarbophos, chlorpyrifos, or triazophos, even at a high concentration of 500 ng/mL. Matrix interference from various agricultural products was also studied in food sample detection. In the spiked test, recoveries of the three OP pesticides ranged from 67 to 120% and relative standard deviations were below 19.54%. These results indicated that the proposed strip assay can be an alternative screening tool for rapid detection of the three OP pesticides in food samples.