文献类型：期刊文献

中文题名：柽柳属植物盐腺的扫描电镜制样方法比较及观察

英文题名：Comparison of sample preparation methods and observation on salt glands of Tamarix using scanning electron microscope

作者：尹丹丹[1] 王留强[2] 曹媛[1] 岳志强[1]

第一作者：尹丹丹

机构：[1]中国林业科学研究院林木遗传育种全国重点实验室,北京100091;[2]中国林业科学研究院林业研究所,北京100091

年份：2024

卷号：43

期号：3

起止页码：354-361

中文期刊名：电子显微学报

外文期刊名：Journal of Chinese Electron Microscopy Society

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD_E2023_2024】；

基金：国家自然科学基金资助项目(No.31971620).

语种：中文

中文关键词：扫描电镜;盐腺;柽柳属;制样方法

外文关键词：scanning electron microscope;salt gland;Tamarix;sample preparation method

分类号：Q94;S722.5;Q336

摘要：为研究柽柳属(Tamarix)植物的盐腺特征,本研究首先采用CO_(2)临界点干燥法和冷冻扫描电镜(Cryo-SEM)制样法两种方法,分别对刚毛柽柳(T.hispida)组培苗和土培苗的叶片进行前处理,并在扫描电镜下观察比较盐腺的形态特征差异。结果发现,对于幼嫩的组培苗,Cryo-SEM制样镜检效果好于CO_(2)临界点干燥制样,盐腺更少变形;对于肉质化的土培苗,CO_(2)临界点干燥制样效果更好,分辨率和衬度较好。接着本研究对10种柽柳土培苗叶片进行了常规干燥制样和扫描电镜观察,并统计了盐腺数量和面积,结果可见长穗柽柳(T.elongata)的盐腺数量和面积均为最高。本研究比较了两种制样法对观察柽柳盐腺形态的优劣且进一步研究了10种柽柳叶片的盐腺,为柽柳抗盐相关基础和应用研究提供一定的参考。
In this study,the leaves of tissue and soil culture seedlings of Tamarix hispida were pretreated by CO_(2) critical point drying method and Cryo-scanning electron microscope(SEM)sample preparation method.The morphological characteristics of salt glands were observed and compared by SEM.The result showed that the effect of Cryo-SEM method was better than that of CO_(2) critical point drying method for young tissue culture seedlings.For fleshy soil culture seedlings,the CO_(2) critical point drying method was better than the Cryo-SEM method.Subsequently,this study conducted the CO_(2) critical point drying method and SEM observation on the leaves of soil culture seedlings of 10 Tamarix species,and counted the number and area of salt glands.The result showed that T.elongata had the highest number and area of salt glands.The advantage and disadvantage of the two sample preparation methods were presented based on the salt gland morphology of Tamarix,and the salt glands of 10 Tamarix leaves were further studied.This work provides a reference for the basic and applied research on salt resistance of Tamarix.