文献类型：期刊文献

英文题名：Allelic variation in the coumarate 3-hydroxylase gene associated with wood properties of Catalpa fargesii Bur.

作者：Lu, Nan[1] Mei, Fang[1] Wang, Zhi[1] Wang, Nan[1] Xiao, Yao[1] Kong, Lisheng[2] Qu, Guanzheng[3] Ma, Wenjun[1] Wang, Junhui[1]

第一作者：卢楠

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Univ Victoria, Ctr Forest Biol, Dept Biol, 3800 Finnerty Rd, Victoria, BC, Canada;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China

年份：2021

卷号：32

期号：6

起止页码：2517-2529

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;Scopus(收录号：2-s2.0-85100407849);WOS:【SCI-EXPANDED(收录号:WOS:000614351100001)】；

基金：This work was supported by the National Key Research and Development Program of China [No.2017YFD0600201] and Forestry Industry Research Special Funds for Public Welfare Projects [No. 201404101].

语种：英文

外文关键词：Catalpa fargesii; Coumarate 3-hydroxylase; Haplotype-based association analyses; Linkage disequilibrium; Single-nucleotide polymorphisms; Wood properties

摘要：Coumarate 3-hydroxylase (C3h) genes participate in the synthesis of lignin and may affect the properties of wood that are important for its commercial value. A better understanding of the natural variation in C3h genes and their associations to wood properties is required to effectively improve wood quality. We used a candidate gene-based association mapping approach to identify CfC3h allelic variants associated with traits that affect the wood properties of Catalpa fargesii. We first isolated the full-length CfC3h cDNA (1825 bp), which was expressed at relatively high levels in xylem according to real time-polymerase chain reaction. In totally, 17 common single-nucleotide polymorphisms (minor allele frequency > 5%) were identified through cloning and sequencing the CfC3h locus from a mapping population (including 88 unrelated natural C. fargesii individuals collected from main distribution area). Nucleotide diversity and linkage disequilibrium (LD) in CfC3h indicate that CfC3h has low nucleotide diversity (pi(t) = 0.0031 and theta(w) = 0.0103) and relatively low LD (within 1800 bp; r(2) >= 0.1). An association analysis identified eight common single-nucleotide polymorphisms (SNPs) (false discovery rate, Q < 0.10) and ten haplotypes (Q < 0.10) associated with wood properties, explaining 4.92-12.09% of the phenotypic variance in an association population consisted of 125 unrelated natural individuals (The 88 individuals from the mapping population were comprised in the association population). Our study would provide new insight into C3h gene affecting wood quality, and the SNP markers identified would have potential applications in marker-assisted breeding in the future.