文献类型：期刊文献

英文题名：Chromosome-Level Genome Assembly of Myricaria laxiflora and Identification of Candidate MylAP2/ERF Genes Involved in Flooding Stress

作者：Xiang, Weibo[1,2,3] Li, Linbao[1,2,3] Li, Guoli[4] Huang, Guiyun[1,2,3] Dun, Bicheng[1,2,3] Wang, Junchen[1,2,3] Chen, Huiyuan[1,2,3] Ma, Xiaobo[1,2,3] Zhang, Haibo[1,2,3] Xiao, Zhiqiang[1,2,3] Liu, Jihong[5] Yang, Zhen[6] Wu, Di[1,2,3]

第一作者：Xiang, Weibo

通信作者：Wu, D[1];Wu, D[2];Wu, D[3];Yang, Z[4]

机构：[1]China Three Gorges Corp, Yangtze River Biodivers Res Ctr, Wuhan 430010, Peoples R China;[2]Hubei Key Lab Rare Resource Plants Three Gorges Re, Yichang 443134, Peoples R China;[3]China Three Gorges Corp, Natl Engn Res Ctr Ecoenvironm Protect Yangtze Rive, Wuhan 430014, Peoples R China;[4]China Agr Univ, Coll Hort, Beijing Key Lab Growth & Dev Regulat Protected Veg, Beijing 100193, Peoples R China;[5]Huazhong Agr Univ, Coll Hort & Forestry, Wuhan 430070, Peoples R China;[6]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China

年份：2025

卷号：16

期号：2

外文期刊名：FORESTS

收录：;EI(收录号：20250917949049);Scopus(收录号：2-s2.0-85218469218);WOS:【SCI-EXPANDED(收录号:WOS:001430764300001)】；

基金：This research was funded by the Natural Science Foundation of Hubei Province of China (2023AFB549), the Yangtze River Hydropower Ecological Environmental Protection Special Fund of China Three Gorges Corporation (WWKY-2021-0131) and the National Natural Science Foundation of China (U2240222).

语种：英文

外文关键词：M. laxiflora; genome assembly; flooding stress; ERF; gene family

摘要：M. laxiflora is an endangered plant that grows in the Yangtze River floodplain of China and often suffers from flooding stress. Due to the lack of a reference genome for M. laxiflora, the molecular regulatory mechanism of waterlogging stress in this plant remains unclear. In this study, we report a high-quality reference genome of M. laxiflora with a size of 1.29 Gb. A total of 23,666 gene-encoding proteins and 5457 ncRNAs were predicted in this reference genome. A comparative genome analysis revealed that 902 and 4299 gene families significantly expanded and contracted, respectively, in M. laxiflora. The expansions of the 902 gene families were significantly related to the "response to stress", "response to abiotic stimulus", and "response to oxygen-containing compounds" pathways. In the M. laxiflora genome, 101 MylAP2/ERF genes were identified and divided into five subgroups. Several MeJA-, ABA-, and hypoxia-responsive elements were found in the promoter regions of these MylAP2/ERF genes. According to the transcriptome data analysis, 74 MylAP2/ERF genes responded to flooding stress. Moreover, three genes (MylAP2/ERF49/78/91) that belong to the same branch as the RAP2.2 gene exhibited different expression trends under flooding stress. Our results provide valuable information on the molecular regulatory mechanism of flooding stress in M. laxiflora.