文献类型：期刊文献

英文题名：Identification of Chalcone Isomerase Family Genes and Roles of CnCHI4 in Flavonoid Metabolism in Camellia nitidissima

作者：Yu, Suhang[1,2,3] Li, Jiyuan[1,3] Peng, Ting[4] Ni, Sui[2] Feng, Yi[1] Wang, Qiushi[5] Wang, Minyan[1] Chu, Xian[1] Fan, Zhengqi[1,3] Li, Xinlei[1] Yin, Hengfu[1] Ge, Wanchuan[1] Liu, Weixin[1]

第一作者：Yu, Suhang

通信作者：Liu, WX[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Peoples R China;[2]Ningbo Univ, Sch Marine Sci, Ningbo 315800, Peoples R China;[3]Jinhua Moxian Hort Engn Co Ltd, Jinhua 321000, Peoples R China;[4]Guizhou Univ, Coll Agr, Guiyang 550525, Peoples R China;[5]Changchun GeneSci Pharmaceut Co Ltd, Changchun 130103, Peoples R China

年份：2023

卷号：13

期号：1

外文期刊名：BIOMOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000917027300001)】；

基金：This work was funded by the Agricultural New Variety Breeding (grant number: 2021C02071-2) from Science and Technology Department of Zhejiang Province, Science and Technology Key Program (grant number: 2022-2-033) from Science and Technology Bureau of Jinhua, Introducing Talents Research Project (grant number: (2016)-45; Natural Science) from Guizhou University, and National Key R&D Program of China (grant number: 2019YFD1001005) from Ministry of Science and Technology of the People's Republic of China.

语种：英文

外文关键词：Camellia nitidissima; chalcone isomerase; flavonoids; CnCHI4; MYB

摘要：Camellia nitidissima is a woody plant with high ornamental value, and its golden-yellow flowers are rich in a variety of bioactive substances, especially flavonoids, that are beneficial to human health. Chalcone isomerases (CHIs) are key enzymes in the flavonoid biosynthesis pathway; however, there is a scarcity of information regarding the CHI family genes of C. nitidissima. In this study, seven CHI genes of C. nitidissima were identified and divided into three subfamilies by phylogenetic analysis. The results of multiple sequence alignment revealed that, unlike CnCHI1/5/6/7, CnCHI2/3/4 are bona fide CHIs that contain all the active site and critical catalytic residues. Analysis of the expression patterns of CnCHIs and the total flavonoid content of the flowers at different developmental stages revealed that CnCHI4 might play an essential role in the flavonoid biosynthesis pathway of C. nitidissima. CnCHI4 overexpression significantly increased flavonoid production in Nicotiana tabacum and C. nitidissima. The results of the dual-luciferase reporter assay and yeast one-hybrid system revealed that CnMYB7 was the key transcription factor that governed the transcription of CnCHI4. The study provides a comprehensive understanding of the CHI family genes of C. nitidissima and performed a preliminary analysis of their functions and regulatory mechanisms.