文献类型：期刊文献

中文题名：毛竹扩展对鹿角杜鹃叶内生真菌群落多样性的影响

英文题名：Effects of Moso bamboo expansion on community diversity of endophytic fungi in leaves of Rhododendron latoucheae

作者：白浩楠[1,2,3,4] 牛香[1,2,3] 王兵[1,2,3] 宋庆丰[1,2,3] 陶玉柱[4]

第一作者：白浩楠

机构：[1]中国林业科学研究院森林生态环境与保护研究所,北京100091;[2]国家林业和草原局森林生态环境重点实验室,北京100091;[3]江西大岗山森林生态系统国家野外科学观测研究站,江西分宜336006;[4]广东省林业科学研究院,广州510000

年份：2021

卷号：40

期号：12

起止页码：3849-3859

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中央级公益性科研院所基本科研业务费专项(CAFYBB2020ZE003);江西大岗山森林生态系统国家野外科学观测研究站(2060503-20-201)资助。

语种：中文

中文关键词：空间代替时间;高通量测序;冗余分析

外文关键词：space instead of time;high-throughput sequencing;redundancy analysis

分类号：S795.7;S685.21

摘要：为探讨毛竹(Phyllostachys edulis)扩展对鹿角杜鹃(Rhododendron latoucheae)叶内生真菌群落多样性的影响,本研究在江西大岗山森林生态站选取受不同扩展程度(未扩展、轻度扩展和重度扩展)影响的鹿角杜鹃叶片为对象,使用高通量测序技术对叶内生真菌进行表征。结果显示:1)毛竹扩展显著增加鹿角杜鹃叶片纤维素含量、木质素含量和叶组织密度,显著降低类黄酮含量;2)不同扩展程度影响下鹿角杜鹃叶内生真菌的类群和相对丰度存在差别,高通量测序共获得5个门、18个纲以及81个属,Ascomycota和Basidiomycota为鹿角杜鹃叶内生真菌优势门菌群,属水平上,g_Hyphoderma、f_Hyphodermataceae、c_Leotiomycetes、o_Helotiales、g_Roussoella、o_Thelephorales、f_Thelephorceae、f_Thyridariaceae、g_Tomentella、f_Diaporthaceae和g_Diaporthe相对丰度变化显著,可作为毛竹扩展影响下的鹿角杜鹃叶内生真菌差异指示种;3)毛竹扩展未影响内生真菌α多样性,主坐标分析显示,毛竹入侵改变了鹿角杜鹃叶内生真菌群落结构;4)冗余分析和Spearman分析表明,纤维素、木质素和叶片韧度对内生真菌群落构建有显著影响。纤维素是毛竹扩展间接影响鹿角杜鹃叶内生真菌群落多样性的重要因子。
We examined the effects of Phyllostachys edulisexpansion on the diversity of endophytic fungal community in leaves of Rhododendron latoucheaein an evergreen forest which were affected by different degrees ofP.edulisexpansion(non-expanded,slightly expanded and severely expanded)in Dagangshan forest ecological station,Jiangxi Province.High throughput sequencing technique was used to characterize endophytic fungi in leaves ofR.latoucheae.We found thatP.edulisexpansion significantly increased leaf cellulose content,lignin content,and leaf tissue density,but significantly decreased flavonoid content ofR.latoucheae.There were differences in the taxa and relative abundance of endophytic fungi in the leaves ofR.latoucheae under different degrees ofP.edulisexpansion.A total of 5 phyla,18 classes and 81 genera were recorded by high-density sequencing.Ascomycota and Basidiomycota were the dominant phyla of endophytic fungi in the leaves ofR.latoucheae.The relative abundances ofG_hyphoderma,F_hyphodermataceae,C_leotiomycetes,O_helotiales,G_roussoella,O_thelephorales,F_thelephorceae,F_Thyridariaceae,G_Tomentella,F_Diaporthaceae,andG_Diaporthechanged significantly,which could be used as the indicator species showing the difference in endophytic fungi ofR.latoucheaeleaves under the influence ofP.edulisexpansion.P.edulisexpansion did not affectα-diversity of endophytic fungi.The results of principal coordinate analysis showed thatP.edulis invasion changed the community structure of endophytic fungi inR.latoucheaeleaves.The results of redundancy analysis and Spearman analysis showed that cellulose,lignin,and leaf toughness had significant effects on community assembly of endophytic fungi.Cellulose is an important factor that indirectly affects the diversity of endophytic fungal community inR.latoucheaeleaves under theP.edulisexpansion.