文献类型：期刊文献

英文题名：Development of male gametophyte of Larix leptolepis Gord. with emphasis on diffuse stage of meiosis

作者：Zhang, Shou-Gong[1] Yang, Wen-Hua[1] Qi, Yu-Chen[2] Li, Mao-Xue[2] Wang, Jian-Hua[1] Sun, Xiao-Mei[1] Wang, Xiao-Shan[1] Qi, Li-Wang[1]

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Cell Biol Lab, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China

年份：2008

卷号：27

期号：11

起止页码：1687-1696

外文期刊名：PLANT CELL REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000260069500001)】；

基金：We are grateful to Guang-Yuan Rao and YanPing Guo for their valuable comments on the manuscript. This work was supported by the National High Technology Research and Development Program of China ( Grant Nos. 2006AA100109, 2007AA021403, 2007AA10Z182 & 2008AA10Z126), the National Natural Science Foundation of China ( Grant No. 30571517), the National "948'' Program ( N0. 2007-4-03).

语种：英文

外文关键词：Diffuse diplotene; Larix; Male gametophyte; Mature pollen; Meiosis

摘要：A basic developmental framework of the Larix leptolepis Gord male gametophyte is presented in detail by squashing technique. The duration of the meiosis stage was more than 6 months, and included a long diffuse stage during winter. This long duration of the diffuse appearance of the diplotene stage makes L. leptolepis a unique suitable experimental material for studying the structure and function of the diffuse stage of meiosis. In particular, the processes of desynapsing and unpairing, which so far have received little attention, can be examined in detail. In L. leptolepis, the chromosomes undergo a dramatic structural reorganization during the diffuse diplotene stage. Based on the clearly visible differences in chromosome morphology, the diffuse diplotene stage was divided into four periods with suggested nomenclature as follows: schizonema, pre-diffuse diplotene, diffuse diplotene and post-diffuse diplotene. Both simultaneous and successive microsporogenesis were observed within L. leptolepis, and there was no strict relationship between the microsporogenesis types and the tetrad configurations, which are strongly influenced by spindle orientation, especially during meiosis II. The mature pollen grain at pollination consists of five cells aligned in an axial row. The prothallial cells cannot be regarded as senescent cells because they remain capable of division.