文献类型：期刊文献

中文题名：马大杂种相思组培快繁技术

英文题名：In vitro propagation of Acacia mangium × A. auriculiformis

作者：施琼[1] 胡峰[2] 黄烈健[1] 陈应彪[3]

第一作者：施琼

机构：[1]中国林业科学研究院热带林业研究所;[2]广东省农业科学院作物研究所;[3]饶平县林业科学研究所

年份：2015

卷号：36

期号：2

起止页码：79-84

中文期刊名：华南农业大学学报

外文期刊名：Journal of South China Agricultural University

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金："十二五"国家科技支撑计划项目(2012BAD01B0402)

语种：中文

中文关键词：马大杂种相思;组织培养;有效增殖倍数

外文关键词：Acacia mangium × A.auriculiformis; tissue culture; proliferation index

分类号：S722.89

摘要：【目的】提高马大杂种相思Acacia mangium×A.auriculiformis良种的快速繁殖效率与推广种植力度.【方法】以马大杂种相思新生枝条带腋芽茎段为外植体,研究马大杂种相思组培快繁体系.【结果和结论】用质量分数为0.1%的升汞和体积分数为75%的乙醇分别处理当年新生枝条第3-5个腋芽茎段15 min和30 s,然后接种至MS培养基进行初代培养,芽诱导率为95.68%;最佳增殖培养基为改良MS＋6-BA 1.5 mg·L^-1＋NAA 0.1 mg·L^-1＋蔗糖30-40 g·L^-1,35 d的有效增殖倍数为3.97;将增殖芽接入含IBA 600 mg·L^-1的MS固体培养基上预培养4-8 h后转接至无激素1/2 MS培养基上,15 d即可全部生根;或将增殖芽直接接入1/2 MS＋IBA 1.0 mg·L^-1＋NAA 0.5 mg·L^-1＋蔗糖30 g·L^-1生根培养基上,第15天时生根率为99.43%.将生根苗移栽至以黄心土为基质的营养袋内,存活率为94.67%.
【Objective】To improve the propagation efficiency and extend the planting of Acacia mangium ×A. auriculiformis.【Method】This study was carried out to explore the techniques of in vitro propagation of A. mangium × A. auriculiformis using stem segments with buds collected from 16-year plants as the explant. 【Result and conclusion】Current season stem segments carrying 3-5 axillary buds were sterilized with w = 0. 1% mercuric chloride and φ = 75% alcoho1 for 15 min and 30 s respectively before they were inoculated onto MS medium. Buds could be induced successfully on MS with a shooting rate of 95. 68%.The bud proliferation index was 3. 97 after being transferred onto MS ＋ 6-BA 1. 5 mg·L^-1＋ NAA 0. 1mg·L^-1＋ sucrose 30-40 g·L^-1for 35 d. The buds all rooted on the 15 thday from inoculation onto1 /2 MS medium after pre-cultured on MS supplemented with IBA 600 mg·L-1for 4-8 h. Inoculating the proliferated buds onto 1 /2 MS ＋ IBA 1. 0 mg·L^-1＋ NAA 0. 5 mg·L^-1＋ sucrose 30 g·L^-1also generated a high rooting rate of 99. 43%. The survival rate reach 94. 67% after the rooted plantlet are transplanted to the yellow soil medium.